摘要
为探索猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)感染猪子宫内膜内皮细胞的机制,研究了CD163分子对PRRSV感染猪子宫内膜内皮细胞的影响。将CD163分子转染猪子宫内膜内皮细胞,构建稳定表达CD163分子的猪子宫内膜内皮细胞系,用0.1 MOI的VR2332株PRRSV感染表达CD163分子的猪子宫内膜内皮细胞,收获不同感染时间的子代病毒并测定病毒滴度。结果显示,同一时间点CD163转染细胞组的PRRSV子代病毒滴度与空载体转染细胞组无明显差异。可见,CD163分子对PRRSV感染猪子宫内膜内皮细胞无明显作用。
In order to investigate the mechanism of porcine endometrial endothelial cells infected by porcine reproductive and respiratory syndrome virus,the effects of CD163 on infection of porcine endometrial endothelial cells by porcine reproductive and respiratory syndrome virus( PRRSV) were studied. The recombinant CD163 plasmid was transfected into porcine endometrial endothelial cells and transgenic cell line was generated. Transcription of CD163 in transgenic cell line was detected by RT-PCR and immuonfluorescence. Transgenic cell line was infected by 0. 1 MOI PRRSV( VR2332 srain). The virus was harvested at different time points post-infection and titrated by Marc-145 cells. The result showed that there was no significant difference between CD163 transgenic cells and vector transgenic cells. In conclusion,CD163 had no significant effection on infection of porcine endometrial endothelial cells by PRRSV.
出处
《河南农业科学》
CSCD
北大核心
2017年第12期130-133,共4页
Journal of Henan Agricultural Sciences
基金
转基因生物新品种培育科技重大专项(2009ZX08010-0198)
江苏高校优势学科建设工程资助项目(PAPD)
