摘要
以生菜种子为试材,采用水培的方法,研究了不同浓度(0、1、5、10、15mg·L^(-1))Cd胁迫对生菜种子萌发、幼苗生长及抗氧化酶的影响。结果表明:随着Cd浓度的升高,生菜种子发芽率、发芽势、发芽指数和活力指数均表现为不同程度的降低,其中高于10mg·L^(-1)胁迫使生菜种子发芽率明显降低,而高于5mg·L^(-1)胁迫使发芽指数和活力指数明显降低。幼苗根、芽长度和鲜质量均表现出低促高抑的效应,高于5mg·L^(-1) Cd胁迫使生菜幼苗根和芽的生长明显受抑。随着镉胁迫浓度的增加,幼根和幼芽中MDA含量、POD活性明显增加,SOD、APX活性则随着镉胁迫浓度的增加表现为先明显增加后略有降低,而CAT活性明显降低。因此,较高浓度镉胁迫造成氧化胁迫是其抑制生菜种子萌发和幼苗生长的重要原因之一。
The hydroponic culture method was adopted to explore the effects of different concentrations of Cd on seed germination,seedling growth and antioxidant enzymes of lettuce.The results showed that the seed germination rate,the germination potential,the germination index and the vigor index were inhibited to different degrees with the increase of Cd concentration.As cadmium concentration increased to 10 mg·L^-1,the germination rate was significantly decreased.However,the germination index and the vigor index were significantly decreased at the cadmium concentration higher than5 mg·L^-1.The length and fresh weight showed low promotion and high inhibitory effect,and markedly inhibited at cadmium concentration higher than 5 mg·L^-1.With the increase of cadmium intimidation,the activity of MDA and POD in the roots and shoots increased gradually,and the activities of SOD and APX increased firstly and then slightly decreased.The CAT activity was significantly reduced in this process.Therefore,the oxidative intimidation caused by the higher concentration of cadmium intimidation is one of the important reasons for inhibiting the seed germination and seedling growth of lettuce.
出处
《北方园艺》
CAS
北大核心
2018年第2期15-21,共7页
Northern Horticulture
基金
国家自然科学基金资助项目(31460100
31660477
31360413
41261095)
关键词
Cd
生菜
萌发
抗氧化酶
Cd
lettuce
germination
antioxidant enzymes
