摘要
为建立一个稳定的高粱遗传转化体系,以高粱RHMC品种为试验材料,采用内含有PCAMBIA3301质粒载体的根癌农杆菌EHA105介导的方法研究了高粱遗传转化的相关因素。结果表明,外植体预培养2d,GUS表达效果最好;在侵染液和共培养基中加入200μmol/L乙酰丁香酮时,GUS表达效果最好;侵染液浓度OD600值的适宜范围为0.8~1.0;农杆菌侵染愈伤组织的适宜时间为5~10min;共培养的适宜时间为2d。初步建立了一套高粱遗传转化体系,为今后高粱转化提供一些参考。
Using sorghum variety RHMC and the agrobacterium strain EHA105 containing pCAMBIA3301 plasmid as the materials, we studied the factors which influence agrobacterium-mediated transformation efficiency of the sorghum so as to establish a stable transformation system. The results showed that when the explants were pre-cultured for 2d, the GUS staining was the best. When 200μmol/L acetosyringone was added to infecting fluid and co-culture media, the GUS staining was the best. The suitable concentration range of the OD600 was 0.8-1.0. The suitable time of the infection with agrobacterium was 5-10min. The suitable time of the co-culture was 2d. In this study, a stable transformation system was preliminary established. This provided some references for the transformation of sorghum in the future.
出处
《作物杂志》
CAS
北大核心
2018年第1期56-61,共6页
Crops
基金
山西省科技攻关项目(20150311003-2)
高粱遗传与种质创新山西省重点实验室青年基金(2015Q-06)
关键词
高粱
农杆菌
遗传转化
愈伤组织
Sorghum
Agrobacterium
Genetic transformation
Callus
