摘要
选用VEG株弓形虫的卵囊和包囊分别灌胃接种昆明小鼠，采用改良凝集试验和PCR方法分别检测小鼠血液中的弓形虫IgG抗体和虫体基图片段。结果显示，卵囊感染的小鼠弓形虫抗体第9天100％检出；而包囊感染的小鼠弓形虫抗体第12天100％检出。VEG株弓形虫感染小鼠后，血液中可检出弓形虫的DNA片段，检出率为25％（6／24）。小鼠组织洗液可检出弓形虫抗体，但组织洗液弓形虫抗体滴度均明显低于血清弓形虫抗体滴度（P〈0．05）。结果表明，小鼠急性感染vEG株弓形虫后，血清学检测方法可靠，在12d可100％检出弓形虫抗体；对于弓形虫弱毒株，PCR方法检测弓形虫核酸片段，检出率低；组织洗液能检测出弓形虫抗体，有望应用于肉类产品的安全性评价。本试验为人类和其他动物弓形虫感染的早期诊断奠定基础。
The aim of this research was to explore the earliest efficient detection time of ToxopIas- ma gondii in early clinical diagnosis of toxoplasmosis in mice infected avirulent strain. The Kun- ming mice were orally inoculated with oocysts or cysts of VEG strain. The IgG antibody and gene of T. gondii in blood were detected by modified agglutination test（MAT） and polymerase chain reaction（PCR）. The earliest detected time of T. gondii antibody（100%） was 9 days post inocula- tion（DPI） oocysts. For cysts,the earliest detected time was 12 DPI. After inoculating VEG strain, the DNA segment of T. gondii was found in the blood. The positive rate was 250%（6/24）. The T. gondii antibody could be found in tissues washed liquid, but the titer was lower than that in the se- rum（P〈0.05）. This research showed that serological method was accurate and 100% detection was 12 DPI in the acute toxoplasmosis of Kunming mice. For the avirulent strain of T. gondii, it is a low positive rate to check the DNA of T. gondii by PCR. The antibody of T. gondii could be found in tissues liquid, and it is useful to monitor the meat safety. The results of this study provide a reference for early diagnosis of toxoplasmosis in animals and human.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第2期359-363,共5页
Chinese Journal of Veterinary Science
基金
国家重点研发计划资助项目(2016YFD0500707)
河南省高校科技创新人才支持计划资助项目(17HASTIT038)
