摘要
目的通过对应用免疫抑制剂的大鼠进行组织移植的方法,建立大鼠荷子宫肌瘤组织移植瘤模型,为研究子宫肌瘤的研究提供新的动物模型。方法术前两周开始,大鼠予免疫抑制剂吗替麦考酚酯(mycophenolate mofetil,MMF)灌胃,持续至整个实验结束。将20只Wistar大鼠随机分为4组,按照腹腔移植术后补充的不同激素分别为A组(芬吗通)、B组(雌二醇)、C组(达芙通)、D组(对照组),术后4周取材,观察肌瘤形态及组织形态学。结果 A组造模成功率90%,B组造模成功率40%,C组、D组造模成功率为0。结论大鼠荷子宫肌瘤移植瘤模型成功率高,成本低,可作为新的研究子宫肌瘤移植瘤的动物模型。
Objective To establish a rat xenograft model of human uterine leiomyoma using immunosuppressive agent and provide a useful tool for the study on uterine leiomyoma. Methods Intragastric administration with immunosuppressive agent mycophenolate mofetil（ MMF）（ 40 mg/kg） was given to rats for two weeks before the surgery until the end of the experiment. 20 SPF female Wistar rats were randomly divided into 4 groups after abdominal transplantation of human leiomyoma tissues： group A received femoston containing 0. 4 mg/kg estradiol and 2 mg/kg dydrogesterone,group B received estadiol 0. 4 mg/kg,group C received dydrogesterone 2 mg/kg,and group D served as the control group,received distilled water 1 m L/200 g. All rat received the corresponding drugs once per day for 2 days. Samples were taken at 4 weeks after the surgery to observe the pathology of the tumor tissues. Results The modeling success rates were 90% in the group A,40 % in the group B,and 0% in the groups C and D. Conclusions Rat xenograft model of human leiomyoma can be successfully established using an immunosuppressive agent femostone with a high modeling success rate and low cost. It can be used as a new animal model for the study of transplanted leiomyoma.
作者
宋阳
甄玉花
关永格
李坤寅
郭宇丹
SONG Yang;ZHEN Yuhua;GUAN Yongge;LI Kunyin;Guo Yudan(Guangzhon University of TCM, Guangzhou, China 510405;The First Affiliated Hospital, Guangzhou University of Chinese Medicine 510405)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2018年第1期91-94,共4页
Acta Laboratorium Animalis Scientia Sinica
基金
广东省自然科学基金(No.2014A030313401)~~
关键词
子宫肌瘤
移植瘤
大鼠
动物模型
uterine leiomyoma
xenografts
wistar rats
model
immunosuppresive agent
mycophenolate mofetil
