摘要
目的:研究长期高脂饮食为主的肥胖个体的肠道细菌多样性,比较不同健康状态的肥胖者肠道菌群的差异。方法:对14个肥胖个体根据健康状况分为3组(健康组,H组;胃肠道疾病组,D组;慢性病组,T组),提取3组样品粪便细菌宏基因组DNA,扩增其16S r RNA基因全长序列,利用三代测序技术测定样品宏基因组DNA的16S r RNA基因全长序列,采用相关生物信息学与统计学方法分析。结果:肥胖个体肠道细菌多样性降低,高丰度细菌含量增加,低丰度细菌含量减少,拟杆菌属是肥胖人群肠道细菌中含量最高的菌属。慢性病肥胖患者肠道菌群的B/F值(拟杆菌门/厚壁菌门)降低,同时产气柯林斯菌和Lachnospira peotinoschiza的相对含量显著高于健康组的肥胖个体(LDA>2)。此外,患有腹泻等胃肠道疾病的肥胖个体(D组)变形菌门下的一些致病菌相对含量增加。结论:肥胖个体的健康状态影响其肠道菌群结构和组成,肥胖引发的不同并发症可能与肠道菌群相关。
Objective： The intestinal bacterial diversity of 14 high-fat diet-induced obese individuals under different health was investigated and the difference of intestinal bacteria from obese individuals of different health condition was compared. Method： 14 obese people were divided into three group according to their health condition（Health group, H group; gut disease, D group and chronic disease, T group）. The DNA was extracted from the stool samples in the 3 groups and the full length 16 S r RNA gene sequences were amplified, then the products of PCR were sequencing using Pacific Biosciences single molecule real-time sequencing technology（Pac Bio SMRT sequencing）. Results： The intestinal bacterial diversity of obese people was decreased, Bacteroidetes and Firmicutes were more abundant, but the low abundance bacteria were more less, and Bacteroides had the highest proportion in core bacteria. The ratio of the relative abundance of Bacteroidetes to that of Firmicutes（B/F ratio） was lower in chronic disease obese group than health group,and the relative abundance of Collinsella aerofaciens and Lachnospira peotinoschiza was significantly higher than obese individuals in health group. In addition, The Parabacteroides was higher in obese individuals of gut disease group. Conclusion： The health condition of obese individuals had an effect on the structure and composition of intestinal bacteria,the complications of obesity may be associated with the changes of intestinal microbial population.
作者
徐海燕
马慧敏
王彦杰
赵飞燕
刘亚华
海棠
张和平
孙志宏
Xu Haiyan;Ma Huimin;Wang Yanjie;Zhao Feiyan;Liu Yahua;Hai Tang;Zhang Heping;Sun Zhihong(Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University, Key Laboratory of Dairy Products Processing, Ministry of Agriculture, Hohhot 010018)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第1期1-11,共11页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(31720103911
31622043)
关键词
肥胖
肠道细菌
高脂饮食
三代测序
obesity
intestinal bacteria
high-fat diet
PacBio SMRT sequencing
