摘要
目的：研究益脾养肝方对二乙基亚硝胺（DEN）诱导的大鼠肝癌癌前病变病理组织和NF-κB、STAT3蛋白表达的影响。方法：将大鼠随机分成对照组、模型组、益脾养肝方大、小剂量组。采用腹腔注射DEN诱发大鼠肝癌前病变模型,给药16周后处死。观察大鼠一般情况和体质量,肝功变化,肝组织HE染色以及免疫组化检测NF-κB和STAT3蛋白表达。结果：与模型组比较,YPYGF大、小剂量组的ALT、AST和GGT均明显降低,其中YPYGF大剂量组效果最佳（P〈0.01）。病理组织结果显示,益脾养肝方可以改善大鼠肝癌癌前病变的病理形态,能显著抑制肝组织NF-κB和STAT3的蛋白表达。结论：益脾养肝方对于大鼠肝癌癌前病变具有抑制作用,其作用可能与抑制炎症因子NF-κB和STAT3表达有关。
Objective： To study the effect of YPYGF on the amelioration of pathological morphology and expression of NF-κB and STAT3 proteins in diethylnitrosamine（DEN） induced rat liver precancerous lesion. Methods： The hepatic precancerous lesion model of male Wistar rats induced by intraperitoneal injection of DEN was used. Rats were randomly divided into the normal control,the DEN group,and two YPYGF（0. 5 g/m L and 1. 0 g/m L） treatment groups. After 16 weeks of administration,rats were sacrificed. We observed generral conditions,body weight and liver function of rats. Liver tissues were measured by HE staining,and the expressions of NF-κB and STAT3 proteins of liver tissues were measured by immunohistochemical detection. Results：Compared with model group,ALT,AST and GGT of two YPYGF groups were significantly lower and the best was high-dose group of YPYGF（P 0. 01）. YPYGF can improve the rat liver precancerous pathological morphology,and can remarkably inhibit the expressions of liver tissue NF-κB and STAT3 proteins. Conclusion： The results suggest that YPYGF significantly suppress precancerous lesion of rats＇ liver by mediating expressions of NF-κB and STAT3 proteins.
出处
《辽宁中医杂志》
CAS
2018年第1期196-198,I0011,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81603612)
陕西省科技厅(2012SF2-11,2014K11-02-04-06,2016SF-234)
陕西省中医药管理局(15-JC009)
北京医学奖励基金会课题(YJHYXKYJJ-276)
