摘要
目的分析我国室内15种常见蜚蠊的细胞色素C氧化酶亚基Ⅰ(COⅠ)基因序列,为建立蜚蠊分子鉴定方法奠定基础。方法通过美国国立生物技术信息中心检索我国室内常见蜚蠊线粒体COⅠ基因序列,应用DNAStar和MEGA 6.0软件进行序列分析,探讨种间差异和遗传关系,利用Neighbor-Joining法构建系统进化树。结果我国室内常见15种蜚蠊种平均进化分歧数为0.166,种间差异较大;系统进化树分析显示,同属蜚蠊COⅠ分子标记分类结果与传统形态分类学基本一致,且可信度均>91。结论基于658 bp COⅠ基因序列,可对我国室内部分蜚蠊进行种下水平的分类鉴定,而该基因对于属或科以上水平的区分及少数近缘种的鉴定有所限制。
Objective To provide basis for future molecular identification methods of cockroach species by analyzing genesequences of cytochrome C oxidase subunitⅠ（COⅠ） among 15 cockroach species. Methods The mitochondrial COⅠgene sequences of cockroaches were searched by NCBI and the sequence alignment was carried out by DNAStar software.An evolutionary divergence and a Neighbor-Joining tree were constructed using MEGA 6.0 software. Results The averageinterspecific genetic distances among 15 species of cockroaches in China was 0.166, and the genetic distances amongspecies were significant. Phylogenetic tree analysis showed that CO Ⅰ molecular markers were the same as traditionalmorphological classification, and the confidentiality more than 91. Conclusion The COⅠ gene can be effectively used todistinguish some species of domestic cockroaches. The value of 658 bp region of the mitochondrial CO Ⅰ gene foridentification of genus or subspecies is limited.
出处
《中国媒介生物学及控制杂志》
CAS
2018年第1期20-22,26,共4页
Chinese Journal of Vector Biology and Control
基金
重大新发传染病综合防控科技示范工程(BE2015714)
江苏省医学重点学科(流行病学)项目(ZDXKA2016008)~~
关键词
蜚蠊
分子鉴定
细胞色素C氧化酶亚基Ⅰ
Cockroach
Molecular identification
Cytochrome C oxidase subunit
