摘要
采用组织块分离法从白木香(Aquilaria sinensis(Lour.)Gilg.)茎秆和叶片中分离得到10株植物内生真菌,并采用滤纸片法检测10种植物内生真菌液体培养粗提物对13种常见致病细菌的抑菌活性.经形态与分子鉴定,白木香中获得的10株内生真菌名称分别为Colletotrichum gloeosporioides、Arthrinium rasikravindrii、Xylaria adscendens、Cladosporium perangustum、Fusarium tricinctum、Cladosporium sphaerospermum、Diaporthe sp.、Phomopsis amygdali、Phomopsis sp.、Periconia macrospinosa.A.rasikravindrii、Diaporthe sp.、P. amygdali、Phomopsis sp.、P. macrospinosa 5株内生菌为首次从白木香中分离得到.活性筛选结果显示有5株内生菌(C. gloeosporioides、C. perangustum、Diaporthe sp.、F. tricinctum、P. macrospinosa)液体培养粗提物具有抗菌活性.其中,Diaporthe sp.对副溶血性弧菌的抑菌圈能达到(17.1±0.04)mm;F. tricinctum粗提物对蜡样芽孢杆菌、无乳链球菌、短小芽孢杆菌、枯草芽孢杆菌、金黄色葡萄球菌、藤黄微球菌、副溶血性弧菌的最低抑菌质量浓度均为3.125 mg·mL^(-1).
The separation and purification of endophytic fungi from the stalks and leaves of Aquilaria sinensis （Lour.） Gilg. were studied by tissue block separation. The antimicrobial activities of the crude extract of endophytic fungi were studied by filter paper method, and the minimum inhibitory concentration （MIC） was determined. The results showed that 10 endophytic fungi were obtained from Aquilaria sinensis （Lour.） Gilg.. The fungi names are as follows： Colletotrichum gloeosporioides, Arthrinium rasikravindrii, Xylaria adscendens, Cladosporium perangustum, Fusarium tricinctum. , Cladosporium sphaerospermum, Diaporthe sp. , Phomopsis amygdali, Phomopsis P. macrospinosa were first seperated from Aquilaria sinensis （ Lour. ） Gilg.. Among extracts of C. gloeosporioides, C. perangustum, Fusarium sp. , Diaporthe sp. and Phomopsis sp., them, the crude P. macrospinosa have antibacterial activitis toward 13 species of test bacteria after the liquid culture. The inhibition zone of Diaporthe sp. toward vibrio parahaemolyticus can reach （17.1 ± 0.04） ram. The minimum inhibitory concentration of crude extract of Fusarium tricinctum, toward Bacillus subtilis, Streptococcus agalactiae, Bacillus subtilis, Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus and Vibrio parahaemolyticus was 3. 125 mg· mL^-1. This study laid the foundation for the development and utilization of antimicrobial compounds in endophytic fungi of Aquilaria sinensis （ Lour. ） Gilg..
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第2期57-64,共8页
Journal of Fujian Normal University：Natural Science Edition
基金
国家自然科学青年科学基金资助项目(31400488)
云南省应用基础研究计划面上项目(2016FB055)
云南省应用基础研究计划青年项目(2014FD069)
关键词
内生真菌
抗菌活性
滤纸片
最低抑菌浓度
白木香
endophytic fungi
antimicrobial activities
filter paper method
MIC
Aquilaria s#~ensis （Lour.） Gilg.
