摘要
以长寿花愈伤组织为外植体,研究了不同激素(6-BA和IBA)浓度配比对长寿花丛生芽诱导以及添加不同浓度NAA对其生根培养的影响。结果表明:长寿花丛生芽诱导增殖的最适培养基为MS+6-BA 1.0mg·L-1+IBA 0.4mg·L-1,诱导率为288.7%;最适生根诱导培养基为1/2MS+NAA 0.3mg·L-1,生根率为63.3%;长寿花生根组培苗成活率较高为97%。
Callus of Kalanchoe blossfeldiana were used as explant.The effects of different hormones（6-BA and IBA）on bud induction and different concentrations NAA on rooting culture of K.blossfeldiana were studied.The results showed that the optimum medium for bud induction and proliferation of K.blossfeldiana was MS＋6-BA 1.0 mg·L-1＋IBA 0.4 mg·L-1,the ratio of induction was 288.7%;the most suitable medium for rooting induction was 1/2 MS＋NAA 0.3 mg·L-1,the rooting ratio was 63.3%.The survival rate of K.blossfeldianaroot plantlets was 97%.
出处
《北方园艺》
CAS
北大核心
2018年第5期99-102,共4页
Northern Horticulture
基金
国家自然科学基金资助项目(31660379)
江西省教育厅科学技术研究资助项目(GJJ151039)
宜春学院大学生创新资助项目(2016035)
关键词
长寿花
组织培养
愈伤组织
Kalanchoe blossfeldiana
tissue culture
callus
