摘要
潲水油一直是关系食品安全的重要问题,本研究旨在建立有效检测潲水油的方法。潲水油中胆固醇的测定方法:首先将油脂用无水乙醇和氢氧化钾于70℃水浴皂化,然后用石油醚提取浓缩,再通过高效液相色谱将甾醇和胆固醇彻底分离,最后在205 nm条件下紫外检测。在该条件下回收率达到75%以上,植物油中胆固醇含量大于0.35 g/L时可以进行准确定量。采用该法进行盲测可以准确判断出模拟潲水油,对市场上的植物油样进行测定,发现有两种油中含有微量的胆固醇。
Hogwash oil is always an important factor concerning food safety, and this study aims to establish an effective method to detect hogwash oil. The determination method of cholesterol in hogwash oil is as follows：first, samples are saponificated with anhydrous ethanol and potassium hydroxide in 70℃ water, and extracted with petroleum ether; then, sterol and cholesterol are separated by high performance liquid chromatography （HPLC）; at last, clear chromatographic peak are observed under 205 nm. By testing concentrated liquid after filtering, extraction rate of cholesterol exceeds 75%, and the accurate and quantitative detection could be obtained when cholesterol content is over 0.35 g/L. This method is used in blind test, and it could accuratelyd etect the simulation pigwash oil, and traces of cholesterol are found in two kinds of vegetable oil collected from the market.
作者
王润华
焦梦悦
张蕴哲
赵艳
Wang Runhua;Jiao Mengyue;Zhang Yunzhe;Zhao Yan(College of Food Science and Technology, Agriculture University of Hebei, Baoding Hebei 071000;College of lnformation Science ＆ Technology, Agriculture University of Hebei, Baoding Hebei 071000)
出处
《中国农学通报》
2018年第9期131-135,共5页
Chinese Agricultural Science Bulletin
基金
河北省高等学校科学技术研究项目"地沟油快速检测生物传感相关技术研究"(ZD20131069)
关键词
胆固醇
潲水油
油脂净化
紫外分光光度法
高效液相色谱法
cholesterol
hogwash oil
oil purification
ultraviolet spectrophotometry
high performance liquid chromatography
