摘要
以玉米自交系Kr701为试验材料,叶面喷施5个不同浓度的冠菌素（COR）,对照为清水,于4℃低温胁迫下培养48 h后取材,研究冠菌素（COR）对低温胁迫下玉米幼苗株高、干鲜重、相对电导率、可溶性蛋白、脯氨酸、相对含水量、SOD（超氧化物歧化酶）、POD（过氧化物酶）、APX（抗坏血酸过氧化物酶）生理生化指标的影响。结果表明,低温会抑制玉米幼苗的正常生长,各个浓度的COR处理均能促进幼苗的生长,缓解叶片相对含水量的降低和电解质的外渗,增加渗透调节物质的含量和抗氧化酶（SOD、POD、APX）的活性,增强活性氧清除能力,以0.01μmol/L处理效果最显著。喷施适宜浓度的COR能通过调控玉米幼苗各个生理生化指标来增强其抗冷性,从而缓解低温对幼苗所造成的伤害。
The experiment was conducted with maize cultivar Kr701. COR solutions of 5 different concentra-tions were sprayed on the leaf surface, the water was control, and the maize was cultivated at low temperature of 4℃ for 48 h. Then studied the effects of COR on plant height, fresh and dry weight, relative electric conductivity, solu-ble protein content, proline, relative water content SOD, POD and APX activity under cold stress. The resultsshowed that low temperature could inhibit the growth of maize seedlings, and different concentrations of COR couldpromote the growth of seedlings, alleviate the decreased of leaf relative water content and the extravasation of elec-trolyte, increase the content of osmotic adjustment and antioxidant enzymes（SOD, POD, APX） activity, enhance theability of active oxygen scavenging, and 0.01 μmol/L was the most effective concentration. Spraying COR with ap-propriate concentration could enhance the cold tolerance of maize seedlings by regulating the physiological and bio-chemical indexes, and thus alleviate the damage caused by low temperature.
作者
杨德光
尉菊萍
樊海潮
刘洋
谷景龙
蒋芳芳
张倩
姜峰
谭伟明
刘怀宇
孙平立
YANG De-guang;YU Ju-ping;FAN Hai-chao;LIU Yang;GU Jing-long;JIANG Fang-fang;ZHANG Qian;JIANG Feng;TAN Wei-ming;LIU Huai-yu;SUN Ping-li(College of Agriculture, Northeast Agricultural University, Harbin 150030;College of Agronomy and Biotechnology, China Agricultural University/ Engineering Research Center of Plant Growth Regulator, Ministry of Education, Beijing 100193, China)
出处
《玉米科学》
CAS
CSCD
北大核心
2018年第2期81-88,共8页
Journal of Maize Sciences
基金
中国博士后科学基金(2015M571383)
黑龙江省博士后资金项目(LBH-Z14028)
农业部农业科研杰出人才及创新团队项目(2016)
关键词
玉米
低温胁迫
冠菌素
生理特性
Maize
Low temperature stress
Coronatine
Physiological property
