摘要
建立了大鼠尿样中调环酸钙的高效液相色谱(HPLC)测定方法。样品经Waters OasisMAX 3cc固相萃取小柱净化,Poroshell 120EC-C18色谱柱(100×4.6 mm,2.7μm)分离,甲醇-0.01%甲酸水溶液梯度洗脱,流速为0.4mL/min,检测波长275nm。研究结果表明,尿样中调环酸钙的质量浓度在0.20~5.00μg/mL范围内线性关系良好(R2=0.999),检出限为0.06μg/mL,定量限为0.19μg/mL,回收率在96.41%~104.94%之间。日内相对标准偏差(RSD)为2.14%~3.89%,日间RSD为5.01%~7.58%。该方法前处理简单可行,适合尿样中调环酸钙的测定。
Prohexadion-calcium was purified by Waters Oasis MAX column （3cc） cartridges and determinated by HPLC-UV. The standard curves for prohexadion-calcium showed good linearity in the range of 0.20-5.00 /ag/mL with the correlation coefficient R2 = 0. 999. The recoveries were 96.41%- 104.94%. The relative standard deviations（RSDs） of the interassys were 2. 14%-3.89% and RSDs of intraassays were 5.01%-7.58%, respectively. The detection limit of prohexadion-calcium was 0.06 μg/mL, and the limit of quantification for prohexadion-calcium was 0. 19 μg/mL. This method was simple and suitable for the determination of prohexadion-calcium in urine.
作者
马安萍
陈红红
阮小林
丘静静
吴邦华
MA An-ping;CHEN Hong-hong;RUAN Xiao-lin;QIU Jing-jing;WU Bang-hua(School of Public Health ,Guangdong Pharmaceutical University ,Guangzhou ,Guangdong 510310;Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, Guangdong 510310)
出处
《分析科学学报》
CAS
CSCD
北大核心
2018年第2期291-293,共3页
Journal of Analytical Science
关键词
尿样
调环酸钙
固相萃取
高效液相色谱法
Urine sample
Prohexadion-calcium=Solid phase extraction
High performance liquid chromatography
