摘要
目的:观察针刺对慢性应激抑郁模型大鼠前额叶皮层核转录因子κB(NF-κB)炎性信号通路中NF-κB、诱导型一氧化氮合酶(iNOS)及一氧化氮(NO)表达的影响,探讨针刺抗抑郁的作用机制。方法:将SD大鼠随机分为空白组、模型组、针刺组、氟西汀组,每组8只。采用28d慢性不可预知应激法建立抑郁模型。针刺组针刺"百会""内关"穴,隔日1次,共14次;氟西汀组予氟西汀灌胃(10mg/kg),1次/d,共28次。观察大鼠糖水摄入量及旷场实验行为;Western blot法检测大鼠前额叶皮层NF-κB的表达水平;ELISA法检测前额叶皮层iNOS表达,硝酸还原酶法检测前额叶皮层NO含量。结果:与空白组比较,造模后大鼠糖水摄入量、旷场实验爬格数与站立次数显著降低(P<0.01),前额叶皮层NF-κB、iNOS、NO表达水平显著升高(P<0.01)。与模型组比较,针刺组及氟西汀组大鼠糖水摄入量、旷场实验爬格数与站立次数显著升高(P<0.01,P<0.05),前额叶皮层NF-κB、iNOS、NO表达水平显著降低(P<0.01)。针刺组与氟西汀组比较差异无统计学意义(P>0.05)。结论:针刺可能通过抑制NF-κB炎性信号通路,下调NF-κB、iNOS、NO的表达水平,从而缓解炎性反应引起的脑损伤,发挥抗抑郁作用,这可能是针刺治疗抑郁症的机制之一。
Objective To observe the effect of acupuncture intervention on depressive-like behavior and nuclear factor-κB（NF-κB）,inducible nitric oxide synthase（iNOS）and nitric oxide（NO）levels in the prefrontal cortex in chronic stress induced depression rats,so as to explore its mechanism underlying antidepressant effect.Methods SD rats were randomly divided into control,model,acupuncture and medication（Fluoxetine）groups（8 rats/group）.The depression model was established by chronic unpredictable stress stimulation（fasting,water deprivation,cold water swimming,tail clamping,constraining,etc.,and solitary raising）for 28 days.Acupuncture treatment was applied to ＂Baihui＂（GV 20）and unilateral＂Neiguan＂（PC 6）once every other day for 14 days.Fluoxetine（10 mg/kg）was given to rats of the medication group by gavage（p.o.）before every stress stimulation,once every day for 28 days.The animals＇ behavior was tested by using sucrose intake and open field tests（number of crossings and rearings in 5 min）.The expression of NF-κB in the prefrontal cortex was detected by Western blot,and the contents of prefrontal iNOS and NO were detected by ELISA and nitric reductases,respectively.Results After modeling,the sucrose intake and the numbers of crossing and rearing times were significantly decreased（P〈0.01）,and the expression of NF-κB protein and the contents of iNOS and NO in the prefrontal cortex were significantly increased in the model group compared with the control group（P〈0.01）.Following the treatment,the sucrose intake and the numbers of crossing and rearing times were significantly increased in both acupuncture and medication groups compared with the model group（P〈0.05,P〈0.01）,and the expression level of NF-κB,and iNOS and NO contents in the prefrontal cortex were significantly lower in both acupuncture and medication groups than in the model group（P〈0.01）.There were no significant differences between the acupuncture and medication groups in the crossing number and rearing times,NF-κB expression,and iNOS and NO levels（P〉0.05）.Conclusion Acupuncture treatment can improve the depressive-like behavior of depression rats,which is closely related to its effects in downregulating the levels of NF-κB protein,iNOS and NO in the prefrontal cortex to reduce brain inflammatory damage.
作者
蒲荣
邵润慧
卢峻
金树英
图娅
PU Rong;SHAO Run-hui;LU Jun;JIN Shu-ying;TU Ya(School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China;Guo Yitang, Hebei Cangzhou Central Hospital, Cangzhou 061000, Hebei Province;Department of Acupuncture and Moxibustion, Beijing Fengtai District Puhuangyu Community Health Center, Beijing 100079)
出处
《针刺研究》
CAS
CSCD
北大核心
2018年第4期226-230,共5页
Acupuncture Research
基金
国家自然科学基金项目(No.81102638)
关键词
慢性应激
抑郁症
针刺
核转录因子ΚB
诱导型一氧化氮合酶
一氧化氮
Chronic stress
Depression
Acupuncture
Nuclear factor-κB （NF-κB）
Inducible nitric oxide synthase（iNOS）
Nitric oxide （NO）
