摘要
目的:建立高效液相色谱法同时测定怀牛膝中β-蜕皮甾酮、25R-牛膝甾酮、25S-牛膝甾酮、人参皂苷Ro和竹节参皂苷Ⅳa的含量。方法:采用ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.5%磷酸溶液(B)为流动相,梯度洗脱,流速1.0 m L·min^(-1),检测波长250 nm(β-蜕皮甾酮、25R-牛膝甾酮和25S-牛膝甾酮)和203 nm(人参皂苷Ro和竹节参皂苷Ⅳa)。结果:5个分析物分离良好,β-蜕皮甾酮、25R-牛膝甾酮、25S-牛膝甾酮、人参皂苷Ro和竹节参皂苷Ⅳa进样量分别在0.218~2.725μg(r=0.999 8)、0.080~0.995μg(r=0.999 9)、0.155~1.935μg(r=0.999 9)、0.352~4.400μg(r=0.999 8)和0.338~4.225μg(r=0.999 8)范围内呈良好的线性关系,加样回收率分别为98.3%、101.5%、98.4%、99.3%和101.4%,相应的RSD分别为0.89%、0.93%、1.4%、0.69%和1.3%;16批不同产地的怀牛膝中β-蜕皮甾酮、25R-牛膝甾酮、25S-牛膝甾酮、人参皂苷Ro和竹节参皂苷Ⅳa的含量范围分别为0.032%~0.074%、0.008%~0.017%、0.008%~0.017%、0.044%~0.107%和0.047%~0.103%。结论:本方法可作为评价怀牛膝质量的方法。
Objective：To establish an HPLC method for simultaneous determination of β-ecdysterone,25 R-inokosterone,25 S-inokosterone,ginsenoside Ro and chikusetsusaponin Ⅳa in Achyranthis Bidentatae Radix. Methods：The chromatographic separation was performed on a ZORBAX SB-C18 column（4.6 mm×250 mm, 5 μm）with the mobile phase consisting of acetonitrile（A）-0.5% phosphoric acid（B）in gradient elution mode at a flow rate of 1.0 mL·min^-1.The detection wavelength was set at 250 nm for β-ecdysterone,25 R-inokosterone and 25 S-inokosterone,and 203 nm for ginsenoside Ro and chikusetsusaponin Ⅳa.Results：The analytes were separated well.The linear ranges of β-ecdysterone,25 R-inokosterone,25 S-inokosterone,ginsenoside Ro and chikusetsusaponin Ⅳa were 0.218-2.725 μg（r=0.999 8）,0.080-0.995 μg（r=0.999 9）,0.155-1.935 μg（r=0.999 9）,0.352-4.400 μg（r=0.999 8）and 0.338-4.225 μg（r=0.999 8）,respectively.The average recovery rates were 98.3%,101.5%,98.4%,99.3% and 101.4%,with RSD of 0.89%,0.93%,1.4%,0.69% and 1.3%,respectively.In Achyranthis Bidentatae Radix from different habitats,the contents of β-ecdysterone,25 R-inokosterone,25 S-inokosterone,ginsenoside Ro and chikusetsusaponin Ⅳa were in ranges of 0.032%-0.074%,0.008%-0.017%,0.008%-0.017%,0.044%-0.107% and 0.047%-0.103%,respectively.Conclusion：The method can be used for quality evaluation of Achyranthis Bidentatae Radix.
作者
张留记
刘晓苗
屠万倩
李向阳
ZHANG Liu-ji;LIU Xiao-miao;TU Wan-qian;LI Xiang-yang(Henan Academy of Traditional Chinese Medicine, Zhengzhou 450004, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan Institute of Food and Drug Control, Zhengzhou 450018, China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2018年第4期623-629,共7页
Chinese Journal of Pharmaceutical Analysis
基金
河南省重点科技攻关计划项目(102102310018)
