摘要
非生物胁迫是影响农作物产量的主要因素，植物对非生物胁迫的应答机制一直是植物学研究的热点之一. 在拟南芥中克隆和鉴定了一个参与非生物胁应答的新基因SI（stress insensitive），该基因编码一个未知功能结构域的蛋白DUF1336，该家族蛋白结构域具有许多未知功能的假设植物蛋白C末端（约250个残基）. 在单子叶植物和双子叶植物中，SI蛋白质是保守的. 通过瞬时转化实验证明，该蛋白定位于细胞质膜. 实时定量PCR分析结果显示，SI基因在各种组织中组成型表达. 在花、果荚和种子中表达量相对较高. 非生物胁迫和脱落酸（ABA）可以增加SI基因的表达，SI基因在ABA、NaCl和冷处理后分别上调3.5倍、2.1倍和4.7倍. SI基因的T-DNA插入突变体（SALK_021811C）植株表现出对冷胁迫和高盐胁迫的抗性. 种子的萌发实验也表明，突变体种子可以在高盐胁迫条件下萌发，对ABA的抑制作用也不敏感. 本研究表明SI基因是植物抗逆性的负调控因子，可为提高农作物的遗传改良提供新的候选基因. （图5 参35）
Abiotic stresses are a major cause of loss of crop yield. The adaptation of plants to abiotic stress has been always a hotspot of botanical research. In this study, we cloned and characterized a novel gene SI （stress insensitive） involved in the response to abiotic stress in Arabidopsis. The SI protein has a new domain called DUF1336, and this family represents the C-terminus （approximately 250 residues） of a number of hypothetical plant proteins of unknown function. Furthermore, the SI protein is conserved among monocots and dicots. The subcellular localization analysis showed that the SI protein is localized in the plasma membrane. The quantitative RT-PCR analysis showed that the SI gene is constitutively expressed in various tissues. The expression of SI was relatively high in flowers, siliques, and seeds. Abiotic stress and abscisic acid （ABA） treatment can enhance the expression of SI gene, and SI transcripts were upregulated under ABA, NaCl, and cold treatments by 3.5, 2.1, and 4.7 folds, respectively. The T-DNA insertion mutant of SI gene （SALK 021811C） showed increased tolerance to cold and salt stresses. In addition, the seed germination experiments indicated that the seeds of SI mutant were insensitive to salt stress and ABA treatment. In conclusion, the results demonstrate that the SI gene plays a negative role in the tolerance of plants to abiotic stress and is a good candidate gene for genetic engineering to increase the tolerance of crop to abiotic stress.
作者
邹婷
刘唤唤
刘永胜
汪松虎
ZOU Ting;LIU Huanhuan;LIU Yongsheng;WANG Songhu(Ministry of Education Key Laboratory for Bio-resource and Eco-environment, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China;Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Nay Pyi Taw 05282, Myanmar)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2018年第2期328-334,共7页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(31400265)
中国科学院百人计划项目
中国科学院东南亚生物多样性研究中心项目(Y4ZK111B01)资助
四川省国际科技合作项目(2017HH0025)~~
