摘要
目的:建立金牛眼药中煅炉甘石和冰片含量测定的方法。方法:采用乙二胺四乙酸配位滴定法测定炉甘石中氧化锌含量;采用DB-WAX石英毛细管柱(30 m×0.32 mm,0.5μm),柱温:150℃,进样口温度:180℃,检测器温度:200℃,分流比:5∶1,氮气流速:1 ml·min^(-1),FID检测器,乙酸乙酯为溶剂,测定冰片含量。结果:氧化锌加样回收率为101.5%,RSD为1.2%(n=9),冰片在0.1~5.0μg进样范围内线性关系良好,r=0.999 9;加样回收率为98.18%,RSD为0.8%(n=9);样品含量测定结果氧化锌为0.38~0.59 g·g^(-1),冰片为0.13~0.21 g·g^(-1)。结论:建立的方法简便、准确,重复性和稳定性良好,可用于金牛眼药的质量控制。
Objective： To develop the methods for the determination of calcined calamine and borneol in Jinniu ophthalmic remedy. Methods ： The content of zinc oxide in calcined calamine was determined by an EDTA coordination titration method. A DB-WAX capillary column （30 m×0.32 mm,0.5μm） was used with an FID as the detector and ethylacetate as the solvent to determine the content of borneol. The column temperature was 150 ℃, the injection port temperature was 180 ℃, the detector temperature was 200 ℃, the split ratio was 5：1 ,and the flow rate was 1 ml · min-1. Results： The recovery of zinc oxide was 101.5% with the RSD was 1.2% （n = 9）. The linear range of borneol was 0.1- 5.0 μg （r = 0.999 9）. The recovery of borneol was 98.18% with the RSD was 0.8% （n = 9）. The content of zinc oxide and borneol in the samples was 0.38- 0.59 g · g-1 and 0.13-0.21 g· g-1, respectively. Conclusion： The method is simple and accurate with high reproducibility and promising stability, which can be used for the quality control of Jinniu ophthalmic remedy.
作者
王常顺
袁浩
刘永利
王晓蕾
杨抒楠
冯丽
Wang Changshun;Yuan Hao;Liu Yongli;Wang Xiaolei;Yang Shunan;Feng Li(Hebei Institute for Drug Control, Shijiazhuang 050011, China)
出处
《中国药师》
CAS
2018年第5期937-939,共3页
China Pharmacist
基金
河北省科技计划项目(编号:17272504D)
关键词
金牛眼药
煅炉甘石
氧化锌
冰片
质量控制
Jinniu ophthalmic remedy
Calcined calamine
Zinc oxide
Borneol
Quality control
