摘要
合理的轮作可以改善土壤微生物群落结构,提高土壤肥力,减少病虫害的发生。为了探讨小麦和玉米轮作对土壤细菌的影响,采用传统PCR克隆文库构建、克隆测序并结合EzBioCloud细菌分类鉴定,对河南小麦-玉米轮作体系下农田土壤细菌的菌群结构变化及多样性特征进行了分析。结果表明,变形菌门(33.73%)和拟杆菌门(31.50%)细菌是小麦-玉米轮作体系土壤中最主要的优势菌群,其次为酸杆菌门、芽单胞菌门、放线菌门和疣微菌门等。在小麦和玉米生长期内细菌的丰度和多样性明显高于二者的间隔期;玉米生长期间细菌菌群丰度和物种多样性更高。不同的微生物群落在土壤中担负不同的生态功能。
Rational rotation can optimize community structure and diversity of soil bacteria,improve soil nutrients and reduce the occurrence of plant diseases. In order to explore the effect of wheat-maize rotation on bacterial communities in the field soil,structure,variation and diversity of bacterial communities were analyzed by constructing clone library of bacterial 16S rRNA gene,and sequencing and EzBioCloud-based taxonomic identification. The results showed that Proteobacteria（33.73%） and Bacteroides（31.50%） were the dominant bacterial populations in the wheat-maize rotation field,followed by Acidobacteria,Gemmatimonadetes,Actinobacteria and Verrucomicrobia. Comparative analysis of three soil samples individually collected from wheat and maize planting periods,and their interval showed that the abundance and diversity of the bacteria were significantly higher in the planting period than in the interval between wheat and maize. Microbial communities play different ecosystem functions in the soil.
作者
郭丰源
王博文
李文鹏
牛秋红
GUO Fengyuan;WANG Bowen;LI Wenpeng;NIU Qiuhong(Zhengzhou Foreign Language School,Zhengzhou,Henan 450006,China;College of Life Sciences and Technology,Nanyang Normal University,Nanyang,Henan 473061,China)
出处
《麦类作物学报》
CAS
CSCD
北大核心
2018年第5期623-630,共8页
Journal of Triticeae Crops
基金
河南省高校科技创新人才支持计划项目(17HASTIT041)
