摘要
目的探索人工真皮在修复全层皮肤组织缺损过程中的作用，并对其引导皮肤附属器再生的功能进行初步研究。方法于SD大鼠背侧皮肤作一3cm×3cm全层皮肤缺损创面，实验组采用人工真皮覆盖创面，对照组采用硅胶膜覆盖创面。术后2周及4周分别观察创面愈合情况以及毛发再生情况，并在此时间点取材后进行HE以及Mason染色，于镜下检测皮肤相关附属器的再生现象。同时采用BrdU（5-溴脱氧尿嘧啶核苷）渗核试验了解创面愈合区域组织是否来源于细胞分裂增殖。结果实验组创面在2周及4周均较对照组有明显缩小，实验组在4周时可见创面愈合区域有毛发生长现象。HE及Masson染色显示新生皮肤区域有毛囊及皮脂腺再生现象。BrdU试验显示实验组中新生皮肤区域的上皮组织、毛囊及皮脂腺内含有大量的Brdu阳性细胞，这也说明了上述组织内的细胞来源于分裂增殖。结论人工真皮在全层皮肤缺损的修复过程中能起到良好的修复作用，不仅能诱导上皮组织的再生，而且能够通过引导皮肤附属器干细胞／前体细胞迁移、增殖及分化达到促进皮肤附属器再生的效果。这一发现将为组织修复以及皮肤器官再生的临床及基础研究提供新的思路。
Objective To explore the function of artificial dermis in repair of full-thickness skin defects, and investigate its potential of inducing skin appendage regeneration. Methods A 3 cm×3 cm full-thickness skin defect was made on the dorsal skin of SD rats. In the experimental group, artificial dermis was used to cover the wound, and the control group was covered with silicon film only. The wotmd healing and hair regeneration were observed at 2 and 4 weeks after the operation. At these time points, HE and Masson staining were performed to detect the regeneration of skin appendages under the microscope. Simultaneously, BrdU （5-bromodeoxyuridine） incorporation test was used to verify whether the tissue of wound healing was derived from cell proliferation. Results The wounds in the experimental group was significantly smaller at 2 and 4 weeks after the operation than those in the control group. In the experimental group, hair growth was seen in the wotmd healing area at 4 weeks postoperatively. HE and Masson staining showed the regeneration of hair follicles and sebaceous glands in the newborn skin area. BrdU test indicated that there were a large number of BrdU positive cells in the epithelial tissue, hair follicles and sebaceous glands of the newborn skin area in the experimental group, which also showed that the cells in the above tissue were derived from division and proliferation. Conclusion Artificial dermis can play a good role in the repair of full-thickness skin defects. It not only can induce the regeneration of epithelial tissue, but also can guide the skin appendages stern/progenitor cell migration, proliferation and differentiation to promote the regeneration of skin appendages. The discovery will provide new ideas for the clinical and basic research of tissue repair and skin regeneration.
作者
陈江海
赵晓博
薛航
周攀
王锟
艾方兴
翁雨雄
Chen Jianghai;Zhao Xiaobo;Xue Hang;Zhou Pan;Wang Kun;Ai Fangxing;Weng Yuxiong(Department of Hand Surgery, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Chin)
出处
《中华手外科杂志》
CSCD
北大核心
2018年第3期228-230,共3页
Chinese Journal of Hand Surgery
基金
国家自然科学基金面上项目（81171194）
关键词
再生
动物
实验
人工真皮
全层皮肤缺损
皮肤附属器
Regeneration
Animals
laboratory
Artificial dennis
Full-thickness skin defects
Skinappendage
