摘要
利用快速溶剂萃取与高效液相色谱技术联用技术,建立了坚果中α维生素E(α-VE)含量的测定方法。以杏仁为例,考察了萃取α-VE的工艺条件,包括萃取溶剂、温度、静态萃取时间和循环次数等。结果表明最佳萃取工艺为:正己烷为萃取溶剂,温度110℃,萃取时间300s,循环2次。分离条件为:以甲醇-水溶液作为流动相进行梯度洗脱,C18色谱柱分离,检测波长:294nm。结果显示,在2～100μg/mL范围内,α-VE的峰面积与其质量浓度线性关系良好(r=0.9999),平均加标回收率为90.1～97.2%,检出限为0.04μg/mL。按照优化后的萃取方法测定了生花生,山核桃,杏仁以及葵花籽4种坚果样品,α-VE的含量为1.82～154.65μg/g。快速溶剂萃取法方法简单,使用溶剂量少,萃取时间短,回收率稳定,满足测定坚果中αVE的检测要求。
The accelerated solvent extraction parameters（extraction solvent,temperature,statictime and cycles）of almonds were optimized in order to maximize the extraction efficiency.The results indicated that the optimum conditions for extraction were as follows：extraction temperature of 110℃,static extraction time of 300 sand static loop of two times while using hexane as the solvent.For the HPLC determination,a C18 column was used,and a mixture of methanol-water was adopted as the mobile phase.The quantification was based on UV detection at 294 nm.The calibration curves ofα-VE was in good linearity over the range of 2-100μg/mL（r=0.9999）,the average recoveries ofα-VE was 90.1%-97.2%and the limit of detection was 0.04μg/mL.Theα-VE contents of peanuts,pecans,almondsand,sunflower seed samples based on the optimized extraction method were 1.82-154.65μg/g.The accelerated solvent extraction is with the advantages of less solvent,less time and stable recoveries,is of celerity and simpleicity for the detection ofα-VE.
作者
郑兆姗
李伟
裴晓华
寿淼钧
刘晓
Zheng Zhaoshan;Li Wei;Pei Xiaohua;Shou Miaojun;Liu Xiao(Beijing Titan Instruments Co. , Ltd. BeUing 100015, China)
出处
《分析仪器》
CAS
2018年第3期77-81,共5页
Analytical Instrumentation
关键词
快速溶剂萃取
液相色谱
坚果
α维生素E
Accelerated solvent extraction
Liquid chromatography （LC）
Nut
α-Vitamin
