摘要
目的探讨雷公藤红素腹腔注射对大鼠卵巢癌移植瘤的抑瘤作用及其可能机制。方法将60只成年F344大鼠随机分为A、B、C组,各20只。三组大鼠均采用皮下接种人卵巢癌HO-8910细胞溶液的方式制作卵巢癌移植瘤动物模型,且均造模成功。造模后B组大鼠腹腔注射顺铂注射液40 mg/(kg·d),C组腹腔注射雷公藤红素40 mg/(kg·d),A组注射同等体积生理盐水,共30 d。给药前、给药后30 d每组各处死10只大鼠,测算肿瘤体积。取给药30 d的大鼠卵巢癌移植瘤组织,采用Western blotting法检测其中的PI3K、p-mTOR、PTEN蛋白。结果与给药前相比,给药后30 d各组大鼠卵巢癌移植瘤体积均大于给药前(P均<0.05);给药后30 d,C组肿瘤体积小于A、B组(P均<0.05),B组肿瘤体积小于A组(P<0.05)。给药后30 d卵巢癌移植瘤组织PI3K、p-mTOR、PTEN相对表达量C组高于B组(P均<0.05),B组高于A组(P均<0.05)。结论雷公藤红素腹腔注射对大鼠卵巢癌转移瘤有抑制作用,且效果优于顺铂。雷公藤红素的抗卵巢癌作用可能与PI3K/mTOR信号通路和PTEN有关。
Objective To study the anti-tumor effect and mechanism of intraperitoneal injection of celastrol on ovarian cancer xenografts in rats. Methods Sixty adult F344 rats were randomly divided into groups A,B and C,with 20 rats in each group. Rats of the three groups were used to make animal models of ovarian cancer xenografts,and all models were successful. After making the models,the rats in the group B were injected with 40 mg/（ kg·D-1） cisplatin injection,and group C was injected with 40 mg/（ kg · D-1） celastrol,and the same volume of normal saline was injected into the rat models of the group A,for 30 d. Ten rats were killed in each group before administration and 30 d after administration,and the tumor volume was calculated. The PI3K,p-mTOR and PTEN proteins of the transplanted tumor tissues of rats were detected by Western blotting. Results The volume of xenografts in each group at 30 d after administration was higher than that before administration（ all P〈0. 05）; at 30 d after administration,the tumor volume in the group C was smaller than that in the groups A and B（ all P〈0. 05）,and the volume of tumor in the group B was smaller than that in the group A（ P〈0. 05）. At 30 d after administration,the relative expression of PI3K,p-mTOR,and PTEN in the ovarian cancer xenograft tissues of group C was higher than that in the group B（ P〈0. 05）,and group B was higher than group A（ P〈0. 05）. Conclusion Intraperitoneal injection of celastrol can inhibit the metastasis of ovarian cancer in rats,its effect is better than cisplatin,and the anti-tumor effect of celastrol may be related to PI3K/mTOR signaling pathway and PTEN.
作者
王诗尧
孙晶
WANG Shiyao;SUN Jing(The Second College of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510000, China)
出处
《山东医药》
CAS
2018年第19期30-32,共3页
Shandong Medical Journal
