摘要
以苦苣为原料,研究其粗多糖的纤维素酶法提取工艺及其体外抗氧化活性。通过单因素试验和正交试验得出,苦苣粗多糖最佳的提取工艺为:纤维素酶的量1.4%、酶解温度50℃、酶解时间1.5 h、料液比1:35,提取率为6.97%。体外抗氧化试验表明:苦苣粗多糖具有一定的体外抗氧化作用,且和其浓度呈正相关。当苦苣粗多糖的浓度为10 mg/mL时,对DPPH·、·OH和·O_2^-的清除率分别为88.2%、83.4%、80.6%。
This paper reported the extraction process of cellulose in crude polysaccharides and its antioxidation activity from Cichorium endivia L. The extraction condition were optimzed by single-factor and orthogonal experiments, the best extraction process of polysaccharide as follows： the cellulose amount was 1.4%, the extraction temperature was 50 ℃, the enzymatic hydrolysis time was 1.5 h, the solid-liquid ratio was 1：35, the extraction yield was 6.97%. The result showed that the Cichorium endivia L polysaccharide had the effect of anti-oxidation in vitro, and it was positively correlated with its concentration. The scavenging effects of DPPH·, ·OH and ·O2- were 88.2%, 83.4%, 80.6% with the concentration of 10 mg/mL.
作者
程丽丽
岳燕霞
CHENG Li-li;YUE Yan-xia(Department of Food Engineering, Luohe Vocational College of Food, Luohe 46200)
出处
《食品科技》
CAS
北大核心
2018年第6期210-214,共5页
Food Science and Technology
关键词
苦苣
粗多糖
提取
抗氧化性
Cichorium endivia L
crude polysaccharide
extraction
antioxidant activity
