摘要
目的传统盖玻片爬片方法在操作过程易出现掉片、碎片等情况,影响实验进程。文中通过介绍一种新的爬片方法可方便实验操作,简化实验环节。方法将1 m L枪头从尾端截取20 mm制作成长筒状腔室备用。中性树胶组:中性树胶黏贴制作好的枪头到载玻片上,成对称双行排列;胶棒加热组:胶棒加热后沿枪头尾端缓慢均匀的涂抹在外侧,尾端朝下粘于载玻片上,成对称双行排列。等待干燥固定后行高压灭菌消毒。分别比较传统盖玻片爬片与用热熔胶制作爬片效果,HE染色观察细胞生长状态。结果胶棒加热组筒体容易脱片,中性树胶组黏附力度更强,在脱胶过程中:中性树胶组去掉枪头后,载玻片上会有中性树胶残留。胶棒加热组钝性分离即可,粘合剂会以胶圈的形式从载玻片上分离。与传统盖玻片爬片法一样,热熔胶制作爬片法制作的爬片装置未发现细胞污染和增殖受限的情况,细胞生长状态良好,分布均匀,无脱胶脱片现象。结论胶棒加热自制获得的爬片装置在实验操作过程中更简单方便,可用于多组别集成爬片。制备过程中的耗材可回收重复利用。
Objective During the process of growing cells on coverslips by traditional method,damages like detachment and fragmentation always happen to slips,which affect the experimental process. The article aimed to introduce a new method of growing cells on coverslips,which facilitated experimental operation and simplified the experiment procedure. Methods 1 mL pipette tips were made into cylindrical chambers for use by cutting 20 mm from the end. Neutral balsam group： The prepared pipette tips were attached onto the slide by neutral balsam in a symmetrical double row. Glue stick heating group： The glue stick was applied slowly and evenly on the outer sides along the end of the pipette tips after it was heated. The ends were glued to the slide with the end downward,forming a symmetrical double-row arrangement. Autoclaving was followed after drying and fixing. A comparison was made between the effects of traditional coverslips and hot-melt coverslips. HE staining was used to observe the cell growth. Results The tubes in glue stick heating group were easy to remove from the slides,while the neutral balsam group showed stronger adhesion. During the removingprocess： there were neutral balsam residues on the slide after the pipette tips were removed in neutral balsam group, while the adhesives could be bluntly removed from the slide in the form of a rubber ring in glue stick heating group. Similar to the traditional method of growing cells on coverdslips,there were no cell contamination or proliferation restriction in the coverslip device made by hot glue. The cells were in good growth and even distribution,without any detachment from the slides. Conclusion The self-made coverslip device by hot-melt adhesive method makes experiment more simplified and convenient,which can be applied in integrated multigroup coverslips. Recycling and reusing of consumables during the preparation process save experiment expenses.
作者
于雷
高俊玲
YU Lei,GAO Jun-ling(School of Basic Medical Sciences,North China University of Scienceand Technology,Tangshan 063000,Hebei,Chin)
出处
《医学研究生学报》
CAS
北大核心
2018年第5期509-511,共3页
Journal of Medical Postgraduates
基金
河北省自然科学基金(H2015209309)
关键词
细胞爬片
细胞培养
染色
cell culture coverslip
cell culture
staining
