摘要
目的探讨线粒体DNA在脓毒血症患者中的发病机制及对血栓形成的影响。方法取2015年8月至2017年3月ICU收治的脓毒症患者123例,设为观察组。根据患者疾病严重程度分为脓毒血症组(n=70)和严重脓毒血症组(n=53)。取同期入院健康体检30例,设为对照组。采用实时荧光定量PCR技术检测各组血浆mt-DNA水平,观察线粒体DNA在脓毒血症患者中的发病机制及血栓形成发生率的影响。结果观察组(脓毒血症组、严重脓毒血症组)线粒体DNA水平,均高于对照组(P<0.05);严重脓毒血症组线粒体DNA水平,高于脓毒血症组(P<0.05);严重脓毒血症组血栓发生率为13.21%高于脓毒血症组的4.28%(P<0.05)。结论脓毒血症患者线粒体DNA水平升高,且与血栓发生率呈正相关。
Objective To investigate the pathogenesis of mitochondrial DNA in sepsis and its impact on the incidence of thrombosis. Methods A total of 123 patients with sepsis in ICU from August 2015 to March-2017 were selected as observation group. The patients were divided into the sepsis group（ n = 70） and the severe sepsis group（ n = 53）. 30 healthy individuals were enrolled in this study,and they were set as control group. The levels of plasma mt-DNA in each group were detected by real-time quantitative PCR technique,To observe the pathogenesis of mitochondrial DNA in patients with sepsis and the influence of thrombosis. Results The level of mitochondrial DNA in observation group（ sepsis group,severe sepsis group）was observed. All of them were higher than the control group（ P〈0. 05）. The level of mitochondrial DNA in severe sepsis group was higher than that in sepsis group（ P〈 0. 05）. The incidence of thrombus in the severe sepsis group was 13. 21% higher than that in the sepsis group 4. 28%（ P 〈0. 05）. Conclusion The level of mitochondrial DNA in the patients with sepsis is higher than that in the sepsis group（ P〈 0. 05）. There was a positive correlation with the incidence of thrombus. and the pathogenesis of DNA in sepsis and its influence on the incidence of thrombosis were analyzed. Results The levels of mitochondrial dna in patients with sepsis were increased,and there was a positive correlation with the incidence of thrombus.
作者
廖小龙
李洁
郭伟新
王中华
覃铁和
LIAO Xiao- long;LI Jie;GUO Wei- xin;WANG Zhong- hua;QIN Tie- he(Guangdong Academy of Medical Sciences ） Geriatrics Research Institute, Guangzhou 510080, China])
出处
《血栓与止血学》
2018年第3期374-376,共3页
Chinese Journal of Thrombosis and Hemostasis
基金
广东省自然科学基金项目2015 A030310455
