摘要
目的：建立了HPLC-DAD法检测中成药及保健食品中16种降糖类化学药物的测定方法。方法：样品经甲醇提取,采用C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇-0.01 mol/L乙酸铵溶液为流动相,梯度洗脱,检测波长210nm、235 nm,外标法定量。结果：16种降糖类化学药物在0.005 mg·m L^-1-0.040 mg·m L^-1浓度范围内线性关系良好,回收率93.1%-108.4%,检出限为0.0003 mg·m L^-1-0.0027 mg·m L^-1。结论：本方法灵敏度高、选择性好、准确度高,可更好的为日常监督检验工作提供技术支持。
Objective： To establish an HPLC-DAD method for the simultaneous determination of sixteen anti-diabetic agents illegally added in traditional Chinese medicines and health food. Methods： Samples were extracted with methanol,and separated by Hplc and quantified with external standard method. A C18 column（250 mm × 4. 6 mm,5 μm） was used,with the mobile phase of methanol and 0. 01 mol/L ammonium acetate by gradient elution. The detection wavelengths were 210 nm and 235 nm. Results：The calibration curves for the sixteen synthetic anti-diabetic agents were linear in the concentration ranges of 0. 005 mg·m L^-1-0. 040 mg·m L^-1 and the recoveries were 93. 1%-108. 4% respectively,and the detection limits of sixteen anti-diabetic agents were 0. 0003 mg·m L-1-0. 0027 mg·m L^-1. Conclusion： The method is sensitive and selective,with excellent accuracy. It can be used for the routine detection in health food.
作者
李荣
卫星华
董靖
LI Rong;WEI Xinghua;DONG Jing(Xi＇an Institute for Food and Drug Control, Xi＇an 710054, Shaanxi, China)
出处
《辽宁中医杂志》
CAS
2018年第5期1022-1024,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(21475024)
关键词
中成药
保健食品
化学降糖成分
高效液相色谱
traditional Chinese patent medicine
health food
anti- diabetic agents
HPLC- DAD
