摘要
磷矿资源日渐枯竭和土壤无效磷素的活化是资源学和肥料学急需解决的重要问题。为了活化土壤磷库资源,解决磷肥大量使用所产生的面源污染问题,采用鉴别培养基从玉米根际土壤中筛选具有防病促生活性的溶磷聚磷菌;采用正交试验,从碳源、氮源及p H 3个方面对溶磷培养条件进行优化。理化和分子鉴定结果显示,分离获得的溶磷菌为Bucillus aryabhattai,命名为XF1;正交试验优化溶磷最佳条件是葡萄糖为碳源,硫酸铵为氮源,p H 5条件下溶磷量最高,达到891.6 mg/L;菌株XF1对Ca_3(PO_4)_2、Al3(PO_4)_2、FePO_4和磷矿粉均有溶解能力;镜检证明菌株XF1内含有poly-P,聚磷率达到21.93%;菌株XF1可产铁载体,抑制人参根腐、返魂草黑斑、禾谷镰孢、串珠镰孢生长;可产吲哚乙酸(IAA),在含色氨酸的培养液中,IAA的浓度达到86.31 mg/L;包裹菌株XF1的玉米种子生长平均株高2.67 cm,平均根长6.5 cm,平均须根数13个,均高于对照。菌株XF1具有较好的溶磷聚磷能力,兼具抗病促生活性,在微生物肥料的开发方面有潜在应用价值。
The depletion of phosphate resources and the activation of insoluble-phosphorus in soils are important issues that urgently need solving in the fields of resource science and fertilizer science.In order to fully mobilize soil phosphorus pool resources and reduce agricultural non-point source pollution caused by application of large amount of phosphorus fertilizer,the initial phosphorus accumulation ability of the isolated strain was determined by using PAM culture medium,disease resistance and growth promoting activity were detected by CAS solid culture,Salkowski colorimetric method,plate paired culture and plant culture.The optimum phosphorus-solubilizing conditions were optimized in 3 aspects of carbon source,nitrogen source and pH value by usingorthogonal tests,the dissolution characteristics of the strain XF1 were determined for a variety of insoluble phosphorus sources.The results of physiological and biochemical tests and 16S rD NA sequence analysis showed that the isolated strain was Bucillus aryabhattai,and was named XF1.The highest soluble phosphorus content was 891.6 mg/L under the optimum conditions of glucose,ammonium sulfate and pH5.Strain XF1 had good solubility inrock phosphorus,the presence of poly-P in the strain XF1 was confirmed by staining microscopy,and the phosphorus accumulating rate reached 21.93%.Strain XF1 can produce iron carrier,which can inhibit the growth of ginseng root rot pathogen,Alternaria solani,Fusarium graminearum and Fusarium oxysporum.The stain can produce IAA,the concentration of IAA reached 86.31 mg/L in the culture medium containing tryptophan,and after germination,the average 2.67 cm height,the average 6.5 cm root length and the average 13 root numbers of the maize seeds wrapped strain XF1 were higher than those in the control.Strain XF1 had a good capacity of phosphorus solubilizing and phosphorus accumulating and disease resistance and growth promoting activity,which qualifies the strain for potential application in the development of microbial fertilizer.
作者
王呈玉
萧龙珍
都郁馨
杨斓
刘淑霞
焉莉
王继红
WANG Chengyu;XIAO Longzhen;DU Yuxin;YANG Lan;LIU Shuxia;YAN Li;WANG Jihong(College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China;Key Laboratory of Sustainable Utilization of Soil Resources in the Commodity Grain Bases in Jilin Province, Changchun 130118, China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2018年第3期290-298,共9页
Journal of Jilin Agricultural University
基金
吉林省科技发展计划项目(20140204043NY)
吉林省科技发展计划项目(20170101004JC)
国家自然科学基金项目(31301850)
关键词
农业面源污染
溶磷菌
溶磷特性
聚磷能力
agricultural non-point source pollution
phosphorus solubilizing bacteria
phosphorus solubilizing characteristic
phosphorus accumulating capacity
