摘要
目的:研究洋金花叶石油醚萃取部位小极性物质的化学成分及含量。方法:洋金花干燥叶用95%乙醇冷浸,得到的浸膏经水分散后分别用石油醚、乙酸乙酯和正丁醇进行萃取。得到的石油醚萃取部分通过正相硅胶柱层析,用二氯甲烷作为流动相进行等度洗脱,得到二氯甲烷的洗脱流分即为小极性成分。通过GC-MS技术对此小极性成分进行分析,并运用气相色谱峰面积百分比法确定其相对百分含量。结果:从洋金花叶石油醚部位共鉴定出了23个成分。结论:本方法适用于洋金花叶石油醚部位小极性成分分析,将为洋金花叶的进一步开发利用提供参考。
Objective： To study the chemical constituents of the petroleum ether extract from the leaves of Datura metel L. Methods： The 95% ethanol extract from the leaves of Datura metel L. underwent silica gel chromatography and was eluted by petroleum ether, ethyl acetate and n - buthanol in turn. The composition of petroleum ether part was separated from the CH2Cl2, and identified by GC / MS, then the relative content of composition was calculated by area normalization method. Results： 23 components were identified, and all of themwere firstly discovered from the petroleum ether part from the leaves of Datura metel L. Conclusion： This research fits into the analysis of low polar components from leaves of Datura metel L. , which provids scientific basis for the further development and utilization of the leaves of Datura metel L.
作者
郭瑞
刘艳
杨炳友
匡海学
GUO Rui;LIU Yan;YANG Bingyou;KUANG Haixue(Shanxi Key Laboratory of Innovative Drug for Serious Diseases Based on Inflammatory Response,Shanxi University of Chinese Medicine,Jinzhong 030619,China;Key Laboratory of Chinese Materia Medica,Ministry of Education,Heilongjiang Key Laboratory of Traditional Chinese Medicine and Natural MedicinePharmacodynamic Material Bases,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《中医药信息》
2018年第3期77-80,共4页
Information on Traditional Chinese Medicine
基金
国家自然科学基金项目(No.81773883)
山西中医药大学博士科研启动基金项目(No.2015BK05)
