摘要
当前莲藕组织培养常以取自田间的藕芽为外植体,但受到取材季节限制和外植体难消毒等因素的影响.对此,本研究以淮北白莲藕种子为实验材料,对种子萌发条件、褐化控制方法、丛生芽诱导和生根培养基等进行优化,拟建立淮北白莲藕快速繁殖体系.结果表明,采用机械破壳后接种于液体培养基可在3 d内实现白莲藕种子萌芽,且萌发率超过90%;在培养基中添加抗氧化剂和活性炭都难以有效控制莲藕褐化,而0.5 g/L植物凝胶则可将白莲藕外植体褐化降低至10%左右;丛生芽诱导最适培养基为MS+0.5 mg/L NAA+1.5 mg/L 6-BA+0.5 mg/L GA3+0.5 g/L植物凝胶,诱导率达68%;1/2 MS液体培养基附加0.5 mg/L NAA生根率达到100%.该研究将有助于淮北白莲藕的脱毒培养复壮及种质资源改良.
To address the contamination control of lotus bud explants sampled from lotus pond,we investigat.ed the germination condition,browning control method and cluster bud induction of Huaibei white lotus rhi.zome,so as to establish the rapid propagation of Huaibei white lotus rhizome. The results showed that physi.cal breaking of seeds and culture in liquid medium achieved germination in three days with ermination ratehigher than 90%. Addition of 0.5 g/L phytagel could effectively control the browning of white lotus rhizomeexplants. The optimal medium for cluster bud induction was MS＋0.5 mg/L NAA＋0.5 mg/L GA＋1.5 mg/L 6-BA＋0.5 g/L phtagel,giving an induction rate of 68%. The optimal rooting medium was 1/2MS supplementedwith 0.5 mg/L NAA,having a rooting rate of 100%. This would be helpful for the virus-free culture of Huai.bei white lotus rhizome and improve its germplasm resources.
作者
赵丰兰
王雷
杨壮
薛建平
段永波
ZHAO Fenglan,WANG Lei,YANG Zhuang,XUE Jianping,DUAN Yongbo(Key Laboratory of Resource Plant Biology of Anhui Province,School of Life Science,Huaibei Normal University,235000,Huaibei,Anhhui,Chin)
出处
《淮北师范大学学报（自然科学版）》
CAS
2018年第2期41-45,共5页
Journal of Huaibei Normal University：Natural Sciences
基金
国家自然科学基金(31501368)
安徽省自然科学基金(1608085MC52)
淮北市科技攻关项目(20150066)
关键词
淮北白莲藕
种子
萌发
茎段
丛生芽
褐化控制
white lotus rhizome；seed；germination；stem segment；cluster buds；browning control
