摘要
目的探讨细胞色素P450 19(CYP19)基因rs10046位点多态性与结直肠癌发生危险性的关系。方法用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)基因分型法,对198例结直肠癌患者(病例组)和309例健康体检者(对照组)的CYP19基因rs10046位点多态性进行检测,比较两组CYP19基因rs10046位点基因型频率及相对危险度并分析其与结直肠癌临床病理特征的关系。结果病例组CYP19基因rs10046位点C/C、C/T、T/T三种基因型频率分别为28.3%、44.4%、27. 3%,对照组分别为17.2%、51. 8%、31.1%;两组间基因型频率比较差异有统计学意义(P <0.05),其中C/T、T/T与C/C比较OR值分别为0.521 (95%CI:0. 330～0.822)和0.532(95%CI:0.322～0. 880)。当按吸烟分层后(将C/T、T/T基因型合并分析),发现不吸烟者C/T、T/T基因型的发病风险显著低于C/C基因型者,OR值0.409(95%CI:0. 210～0.798),而吸烟者没有上述的保护性关联。结论 CYP19基因rs10046位点基因型分布与结直肠癌发病风险有一定关联,突变基因型C/T、T/T结直肠癌的发病风险降低。
Objective To investigate the relationship between the polymorphism of cytochrome P450 19 （CYP19） gene rs10046 and the risk of colon and rectal cancer. Methods Using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）to analyze geue polymorphism in CYP 19 gene rslO046 in 198 cases of colon and rectal cancer patients （case group）and 309 cases of healthy con- trois （control group）. The genotype frequency and relative risk of CYP19 gene rsl00d6 between the two groups were compared and the relationship with the clinicopathological features of colorectal cancer was ana- lyzed. Results In case group,the prevalence rates of CYP19 rs10046 genotypes C/C,C/T and T/T were 28.3% , 44. 4% and 27.3% ,respectively,and 17.2% , 51.8% and 31.1% in the control group,respec- tively, with statistical significant difference （ P 〈 0. 05 ）. Compared with wild-type C/C, the susceptibility of colorectal cancer with the genotypes of C/T and T/T was decreased by 0. 521 （95% CI：0. 330 -0. 822） and 0. 532 （95 % CI：0. 322 -0. 880） respectively. Moveover,in the non-smoking group, the risk of colorec- tal cancer with genotype T/T or C/T was decreased by 0. 409 （ 95% CI： 0. 210 - 0. 798 ） compared with genotype C/C. The interaction was not exist in smoking group. Conclusions The polymorphism of CYP19 gene rs10046 is related to the susceptibility of colon and rectal cancer. The T/T, C/T genotype of CYP19 rs10046 decrease the risk of colon and rectal cancer, and which might be the protective factor of colon and rectal cancer.
作者
郑利军
齐彩霞
马骥
邢晋梁
熊在平
杨智文
朱壮彦
Zheng Lijun1; Qi Caixia2;Ma Ji1; Xing Jinliang1; Xiong Zaiping1;Yang Zhiwen1; Zhu Zhuangyan2(1.Department of General Surgery, the Third People＇s Hospital of Datong, Datong 037008, China ; 2.Department of lmmunology, Medical School of Shanxi Datong Uni- versity, Datong 037009, China)
出处
《中国医师杂志》
CAS
2018年第7期1002-1005,共4页
Journal of Chinese Physician
基金
大同市科技攻关社会发展项目(2016079)~~
