摘要
建立了检测鸡肉中甲硝唑MNZ、二甲硝唑DMZ及其代谢物羟基甲硝唑MNZOH(甲硝唑代谢物)、1-甲基-5硝基-2-羟甲基咪唑HMMNI(二甲硝唑代谢物)残留的超高效液相色谱-串联四级杆质谱(UPLC-MS/MS)残留分析方法。样本在乙腈水溶液提取下经PRIMEHLB固相萃取柱净化,氮吹浓缩后定容过膜上机检测。在1.00~100.0μg/L线性范围内,甲硝唑、二甲硝唑、羟基甲硝唑、羟甲基甲硝咪唑的标准曲线相关系数分别为r=0.99881、r=0.99694、r=0.99975、r=0.99713,方法检出限分别为0.128μg/kg、0.123μg/kg、0.183μg/kg、0.158μg/kg。加标回收试验中甲硝唑、二甲硝唑、羟基甲硝唑、羟甲基甲硝咪唑的加标浓度分别为1.00、2.00、10.0μg/kg,回收率范围分别为81.5%~105.0%,84.5%~102.0%,87.0%~104.0%,87.0%~106.0%。此方法适用于鸡肉中甲硝唑、二甲硝唑及其代谢物残留的检测。
A method for the residue analysis of metronidazole （MNZ）, dimetridazole（DMZ）, Hydroxyl metronidazole（MNZOH） and 2-hydroxymethyl -lmethyl5-nitroimidazole （HMMNI） in chicken was established by ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. The samples were purified by PRIME HLB solid phase extraction column under the extraction of acetonitrile water. After drying and concentrating, the samples were reused and were tested over the membrane. In the linear range of 1.00-100.0μg/L, the correlation coefficient of the standard curve of MNZ, DMZ, MNZOH and HMMNI was r=0.99881, r=0.99694 r=0.99975, r=0.99713, the detection limits were 0.128 μg/kg, 0.123 μg/kg, 0.183 μg/kg, 0.158 μg/kg respectively. The recovery rate of MNZ, DMZ, MNZOH and HMMNI respectively was 81.5 %-105.0 %, 84.5 %-102.0 %, 87.0 %-104.0 %, 87.0 %-106.0 % in the adding standard recovery test when the Standard concentration respectively were 1.0, 2.0 and 10.0μg/kg. This method is suitable for the multi-residue of MNZ, DMZ and their metabolites in chicken.
作者
丁燕玲
陈彤
黄婷
钟名琴
吴雯娟
罗燕
Ding Yanling, Chen Tong, Huang Ting, Zhong Mingqin, Wu Wenjuan, Luo Yan(Shenzhen Agricultural Product Quality Safety Inspection Testing Center, Shenzhen 518055, Chin)
出处
《广东化工》
CAS
2018年第13期245-248,252,共5页
Guangdong Chemical Industry
关键词
甲硝唑
二甲硝唑
代谢物
高效液相色谱-串联质谱
Metronidazole
Dimetridazole
metabolites
ultra-high performance liquid chromatography-tandem mass spectrometry
