摘要
酰基-ACP硫酯酶(fatty acyl-ACP thioesterase,FAT)是控制植物种子油脂合成的关键酶,根据其氨基酸序列和底物特异性不同可分为FATA和FATB两类。为了更深入了解花生FAT(Ah FAT)的特点与功能,本研究对Ah FAT家族基因进行了全基因组生物信息学分析。在花生基因组中共有20个FAT基因,不均匀分布在2个基因组的12条染色体上。20个Ah FATs可以分为Ah FATA和Ah FATB两个亚家族,Ah FATA亚家族有2个基因,Ah FATB亚家族有18个基因。Ah FATA家族基因具有6或8个外显子,Ah FATB家族基因结构较为复杂,外显子数目5~8个;Ah FATs都不具有跨膜结构域但是都具有保守的Acyl-ACP_TE结构域。对Ah FAT家族基因进行可变剪接分析,发现只有少数发生了可变剪接,且具有组织特异性。对Ah FATs表达模式分析的结果显示,Ah FATAs和Ah FATBs都在种子发育后期表达量较高。
Fatty acyl-ACP thioesterase （FAT）is the key enzyme regulating the synthesis of plant seed oil.According to the amino acid sequence and substrate specificity,FAT is divided into FATA and FATB sub-family.In order to better understand the characteristics of AhFAT gene family,the bioinformatics analysis was carried out.There were 20 AhFAT genes in the peanut genome,which heterogeneously distributed on 12 chro-mosomes in the two genomes.The twenty AhFAT genes could be divided into AhFATA and AhFATB subfamily, which had 2 and 18 members respectively.The AhFATA subfamily owned 6 or 8 exons,and the AhFATB sub-family had complex gene structures with 5 ～8 exons.None of the AhFATs had transmembrane domains,but they all owned the conserved Acyl-ACP_TE domain.The alternative splicing analysis of AhFAT gene family revealed that only a few AhFATs undergone alternative splicing and they had different splicing types in different tissues.The AhFATs expression pattern analysis results showed that both AhFATAs and AhFATBs expressed at higher levels in the later stage of seed development.
作者
张会
单雷
李新国
郭峰
孟静静
万书波
彭振英
Zhang Hui;Shan Lei;Li Xinguo;Guo Feng;Meng Jingjing;Wan Shubo;Peng Zhenying(Biotechnology Research Center,Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement,Ecology and Physiology,Jinan 250100,China;Shandong Academy of Agricultural Sciences,Jinan 250100,China)
出处
《山东农业科学》
2018年第6期19-26,共8页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(31571605)
国家科技支撑计划项目(2014BAD11B04)
国家国际科技合作专项(2015DFA31190)
国家现代农业产业技术体系建设专项(CARS-13)
