摘要
目的本研究旨在探讨健脾清热活血法对萎缩性胃炎(CAG)大鼠相关炎症通路因子的影响。方法将CAG大鼠随机分为6组:空白组、模型组、胃萎清高、中、低剂量组及叶酸组。以N-甲基-N′-硝基-N′-亚硝基胍(MNNG)合饥饱失常法造CAG大鼠模型,观察各组大鼠NF-κB炎症通路各因子的变化。结果胃萎清可在一定程度上抑制TNF-α的表达,对炎症的发生起到一定抑制作用。模型组IKK蛋白表达水平明显高于胃萎清高、中、低剂量组及空白组。胃萎清高、中、低剂量组IKK的表达水平与空白组相当,提示胃萎清能在一定程度上抑制炎症因子的表达,从而阻断炎症的发生发展过程。胃萎清高剂量组可下调p50蛋白的表达水平。结论胃萎清能使NF-κB炎症通路的致炎因子TNF-α、IKK、p50的表达水平下降从而起到控制CAG的发展。
Objective The aim of this study is to investigate effects of the Chinese medicine prescription of invigorating the spleen and clearing heat and activating blood on the inflammatory pathway factors in rats with chronic atrophic gastritis（CAG）. Methods CAG rats were randomly divided into 6 groups： control group,model group, Weiweiqing high dose, middle dose and low dose groups, and folic acid group. The CAG rat model was made with N-methyl-N′-nitro-N′-nitroguanidine（MNNG） and hunger satiety method, and the changes of various factors in the inflammatory pathway of NF-κB in rats were observed in each group.Results Weiweiqing could inhibit the expression of TNF-α to a certain extent and inhibit the occurrence of inflammation. The expression level of IKK protein in model group was significantly higher than that of Weiweiqing high, medium and low dose groups as well as blank group. The expression levels of IKK protein in control group corresponded with Weiweiqing group. Weiweiqing could inhibit the inflammatory factors in a certain extent, thereby blocking the development of inflammation. The expression of P50 protein was downregulated in the high dose group. Conclusion Weiweiqing can reduce the expression levels of inflammatory cytokines TNF-α, IKK and P50 in the inflammatory pathway of NF-κB, thereby controlling the development of CAG.
作者
庄昆海
李海文
刘洪
潘静琳
刘凤斌
ZHUANG Kun-hail;Ll Hai-wen;LIU Hong;PAN Jing-lin;LIU Feng-bin(The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 5113000,China)
出处
《现代消化及介入诊疗》
2018年第2期145-148,共4页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
广东省中医药局建设中医药强省专项资金中医优势病种突破项目(粤中医函[2015]19号)
广东省中医药局科研项目(20161132)
广东省中医药强省建设专项资金第二批名中医师承项目(粤中医办函[2015]93号)
关键词
健脾清热活血法
萎缩性胃炎
胃萎清
炎症通路因子
NF-ΚB
Invigorating spleen
clearing heat and activating blood therapy
Chronic atrophic gastritis
Weiweiqing
Inflammatory pathway factors
NF-kB
