摘要
目的观察柯里拉京(Corilagin)对人脑胶质瘤细胞U251生物学特性的影响。方法体外培养U251细胞,应用200μg/ml的柯里拉京分别干预U251细胞24、48和72h后收集细胞,应用Annexin V-FITC细胞凋亡检测试剂盒检测药物干预前后细胞凋亡活性变化;细胞划痕实验检测200μg/ml Corilagin对U251细胞迁移能力的影响。结果 Corilagin可促进U251细胞的凋亡,U251细胞凋亡率随着培养液中Corilagin培养时间的延长而增加(P<0.05);Corilagin可抑制U251细胞的迁移,200μg/ml Corilagin的培养液培养24、48和72h后U251细胞迁移度均低于对照组(P<0.05)。结论 Corilagin对U251细胞有显著的体外细胞毒性作用,能有效促进U251细胞的凋亡和减弱U251细胞的迁移,但其具体的作用机制还有待进一步研究。
Objective To study the effect of corilagin on the biological characteristics of human glioma U251 cells.Methods Glioma U251 cells were cultured in vitro.The cells were intervened by corilagin of 200μg/ml concentrations at different time points （24,48,72h）.Then the cells were collected to detect the change of cell apotosis with Annexin V-FITC Apoptosis Detection Kit.The effect of 200μg/ml corilagin on cell migration was assessed by cell scuffing test.Results Corilagin can promote the apoptosis of U251 cells.With the extension of Corilagin culture time,the apoptosis of U251 cells increased significantly showing a large number of scattered cell debris,and the apoptosis rate of U251 cells increased with the increase of the culture time of Corilagin （P〈0.05）; Corilagin can inhibit the migration of U251 cells.The migration of U251 cells after 200μg/ml Corilagin cultured for 24h,48h and 72h was lower than that of the control group （P〈0.05）.Conclusion Corilagin has significant cytotoxic effect in vitro on U251 cells,which can effectively promote the apoptosis of U251 cells and weaken the migration of U251 cells,but its specific mechanism of action remains to be further studied.
作者
周广洲
陈俊
靳峰
ZHOU Guangzhou;CHEN Jun;JIN Feng(Jining Medical University,Jining 272067,China;The Affiliated Hospital of Jining Medical University,.lining 272029,China)
出处
《济宁医学院学报》
2018年第4期246-249,共4页
Journal of Jining Medical University
基金
国家自然科学基金资助项目(8107779)
山东省自然科学基金(ZR2016HL34)
山东省优秀中青年科学家奖励基金(BS2010YY006)
山东省高等学校科技计划项目(J16LL05)
