摘要
为重金属污染土壤净化及其开发利用提供科学依据,以徐州菊芋、潍坊菊芋为对象进行土培,研究镉(Cd^(2+))不同浓度(0.0mmol/L、0.10mmol/L、0.60mmol/L、1.20mmol/L)胁迫7d、14d、21d后菊芋根际土壤过氧化氢酶、土壤磷酸酶和土壤pH的变化,分析菊芋对镉的富集能力及其生理响应。结果表明:土壤加入低浓度重金属镉后,前期土壤过氧化氢酶活性上升,呈现出一定激活效应,但随着镉浓度的增加及胁迫时间延长呈抑制作用;土壤磷酸酶活性在前、中期均增大,随胁迫时间延长其活性被抑制;徐州菊芋、潍坊菊芋土壤酶活性的变化一致,其中徐州菊芋的适应能力更强;菊芋体内的镉含量随胁迫程度加深总体呈逐渐上升趋势,其分布特征为地下部分>地上部分,且转运系数均小于1,地上部分镉分布徐州菊芋为茎>叶,潍坊菊芋在中低浓度下为叶>茎;菊芋根系的富集系数均大于1。徐州菊芋、潍坊菊芋均表现出较好的耐镉性和富集能力。
The Cd^2+ enrichment capacity and physiological response of two Helianthus tuberosus varieties from Xuzhou and Weifang were analyzed under four Cd^2+ concentrations (0.0 mmol/L, 0.10 mmol/L , 0.60 mmol/L and 1.20 mmol/L) after 7 d, 14 d and 21 d by determining catalase and phosphatase activity and pH of rhizosphere soil around H. tuberosus to provide the scientific basis for purification and utilization of heavy metal contamination soil. Result: The catalase activity of rhizosphere soil around H. tuberosus rises at the earlier stage after the Cd^2+ solution with a lower concentration is added into the soil but is inhibited with increase of Cd^2+ concentration and stress days. The phosphatase activity of rhizosphere soil around H. tuberosus at earlier and medium stage rises but is inhibited with increase of stress days. There is no difference in soil enzyme activity between H. tuberosus variety from Xuzhou and from Weifang and the adaptive capacity of H. tuberosus variety from Xuzhou is higher than H. tuberosus variety from Weifang. The Cd^2+ content in H. tuberosus plants shows a gradual rising trend with increase of Cd^2+ stress degree overall. The distribution feature is underground part 〉 overground part and their transfer coefficients both are less than 1. The Cd^2+ distribution in overground part of H. tuberosus variety from Xuzhou is stem 〉 leaf and the Cd^2+ distribution in overground part of H. tuberosus variety from Weifang is leaf 〉 stem under lower and medium Cd^2+ concentration. The Cd^2+ enrichment coefficient of H. tuberosus roots is more than 1. Two H. tuberosus varieties both have a better resistance to Cd^2+ and Cd^2+ enrichment capacity.
出处
《贵州农业科学》
CAS
2018年第7期77-81,共5页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目(41571049)
2016年浙江省新苗人才计划项目"镉胁迫下菊芋富集调控以及综合机理的探究"(2017R404020)
关键词
菊芋
镉胁迫
富集
根际土壤
Helianthus tuberosus
cadmium stress
enrichment
rhizosphere soil