摘要
目的研究马归液对腺性膀胱炎模型大鼠转化生长因子-β1(transforming growth factor-β1,TGF-β1)、表皮生长因子-β(epidermal growth factor-β,EGF-β)的影响机制。方法采用膀胱灌注DH5α大肠杆菌溶液的方法建立腺性膀胱炎大鼠模型,将模型大鼠分为模型组(灌注生理盐水,1周1次)、马归液1组(灌注马归液,1周1次)、马归液2组(灌注马归液,1周2次)、吡柔比星组(灌注吡柔比星,1周1次),连续干预8周后对大鼠膀胱进行病理学检查,免疫组化法检测TGF-β1、EGF-β的表达。结果病理学显示马归液1组和马归液2组大鼠的膀胱黏膜恢复正常。与模型组比较,吡柔比星组、马归液1组和马归液2组TGF-β1、EGF-β的平均吸光度值、蛋白表达明显升高(P<0.01)。与吡柔比星组比较,马归液1组TGF-β1平均吸光度值、蛋白表达降低、EGF-β平均吸光度值、蛋白表达与吡柔比星组相近,差异均无统计学意义(P>0.05);与吡柔比星组比较,马归液2组TGF-β1平均吸光度值、蛋白表达与吡柔比星组相近,差异无统计学意义(P>0.05),但马归液2组EGF-β平均吸光度值、蛋白表达高于吡柔比星组,差异有统计学意义(P<0.01或P<0.05)。与马归液1组比较,马归液2组TGF-β1、EGF-β的平均吸光度值、蛋白表达明显升高,差异有统计学意义(P<0.05或P<0.01)。结论马归液能促进腺性膀胱炎大鼠膀胱炎性组织的修复,其机制可能与马归液对TGF-β1、EGF-β的调控作用有关。
Objective To study- the mechanisnl of the effect of Magui Liquid on the expression of transforming growth tactor-β11（TGF-β11） and epidermal growth tactor-β1（EGF-β1） in a rat model of glandular cystitis. Methods A rat model of glandular cystitis was established by- bladder intusion with Esclwrichia coli strain DH5α solution. The rats were divided into model group （infusion with saline once a week）, Magui Liquid 1 group （infhsion with Magui Liquid once a week）, Magui Liquid 2 group （infusion with Magui Liquid twice a week）, and pirarubicin group （infhsion with pirarubicin once a week）. The rats received pathological examinations after 8 continouse weeks of drug intervention, Immnunohistochemistty- was used to measure the expression of TGF-β1 and EGF-β. Results Pathological examinations showed that the bladder mucosa returned to normal in the Magui Liquid groups. The pirarubicin group and the Magui Liquid groups had significantly higher mean absorbance and expression levels of EGF-β and TGF-β1 than the model group （P〈0.01）. There were no significant differences in mean absorbance or expression levels of EGF-β or TGF-β1 between the pirarubicin group and the Magui Liquid 1 group （P〉0.05）. There were also no significant difterences in mean absorbance or expression level of TGF-β1 between the pirarubicin group and the Magui Liquid 2 group （P〉0.05）. However, the Magui Liquid 2 group had significantly higher mean absorbance and expression level of EGF-βthan the pirarubicin group （P〈0.01）. The Magui Liquid 2 group had significantly higher mean absorbance and expression levels of EGF-β and TGF-β1 than the Magui Liquid lgroup （P〈0.05 or P〈0.01）. Conclusion Magui Liquid can promote the repair of the inflammatory tissue in rats with glandular cystitis, probably by regulation of TGF-β1 and EGF-β.
作者
蔡蔚
谢海平
龙蠡
谢晓
杨华伟
CAI Wei;XIE Haiping;LONG Li;XIE Xiao;YANG Huawei(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China)
出处
《湖南中医药大学学报》
CAS
2018年第8期870-874,共5页
Journal of Hunan University of Chinese Medicine
基金
湖南省中医药管理局重点项目(201728)
湖南省教育厅优秀青年项目(15B175)
湖南省省级教学规划课题(XJK015BGD049)
关键词
马归液
腺性膀胱炎
转化生长因子Β1
表皮生长因子β
Magui Liquid
glandular cystitis
transtorming growth tactor-β1
epidermal growth tactor-β
