摘要
本实验检测了暴露于不同浓度多环芳烃(PAHs)中菲律宾蛤仔(Ruditapes philippinarum)鳃丝和消化盲囊的几项解毒代谢指标(AHH、GST、GSH、SOD)和观察了DNA损伤的变化。苯并(a)芘{B[a]P}的浓度设置为0、0.01、0.2μg/L。结果表明,B[a]P对菲律宾蛤仔鳃丝和消化盲囊AHH、GST、SOD等酶活力和GSH含量均具有显著的诱导作用(P<0.05),并且呈现出明显的时间和剂量效应特征。鳃丝和消化盲囊中各指标具有相似的变化规律:在染毒实验过程中,AHH活力被诱导升高,被诱导程度与暴污浓度呈现正相关;GST活力和GSH含量先表现为被诱导升高,之后被抑制并且被抑制程度与B[a]P浓度相关;SOD活力呈现一定的峰值变化,在3 d时达到最大值,之后被抑制;DNA单链断裂水平也被显著诱导,被诱导程度与暴污浓度呈现正相关。暴露于PAHs的菲律宾蛤仔鳃丝和消化盲囊的AHH、GST、SOD活力和GSH含量的变化反映了机体的解毒代谢过程和能力,而DNA单链断裂水平则直接反映了机体的氧化损伤程度,两者构成综合生物标志物,可准确反映PAHs对菲律宾蛤仔的毒性效应。
This study examined several metabolic detoxification indexes（AHH,GST,GSH and SOD） and the change of DNA damage in the Philippines clam（Ruditapes philippinarum） gill filament and digestive blind sac exposed to different concentrations of PAHs. The concentration of B[a]P was set to be 0,0. 01,and 0. 2 ug/L. The results showed that B[a]P had a significant induction effect on the AHH,GST,GSH and SOD activity of gills and digestive blind sacs of R. philippinarum（P 0. 05）,and showed obvious characteristics of time and dose effect. All the indicators of branchial filaments and digestive blind sacs had similar change rules. During the exposure experiment,AHH activity was induced to increase,and the degree of induction was positively correlated with the concentration of violent pollution. GST and GSH activity were firstly induced to increase,then inhibited and correlated with B[a]P concentration. The activity of SOD showed a certain peak change,reaching the maximum in 3 days,and then was inhibited. The level of DNA single chain fracture was also significantly induced,and the degree of induction was positively correlated with the pollution concentration. Exposure to PAHs affected the AHH,GST,GSH and SOD activity in the R. philippinarum gill filaments and digestive blind sac,and then reflected the ability of the body＇s metabolism and detoxification,while the DNA level of single fracture directly reflected the oxidative damage degree of the body.Both of these constitute a combination of biological markers,can accurately reflect the toxicological effect of PAHs to the R. philippinarum.
作者
卓艺蓉
ZHUO Yirong(Pingtan Comprehensive Experimental Area Marine and Fishery Law Enforcement Branch,Pingtan 350400,Chin)
出处
《渔业研究》
2018年第4期268-278,共11页
Journal of Fisheries Research
