摘要
目的探讨富血小板纤维蛋白(platelet rich fibrin,PRF)形态学超微结构及意义。方法选择10例健康志愿者,抽取静脉血制备PRF,对PRF分别行光学显微镜、扫描电子显微镜(scanning electron microscope,SEM)和透射电子显微镜(transmission electron microscope,TEM)观察。结果 PRF为淡黄色纤维蛋白凝胶,H-E染色见胶原纤维为染色均一的淡红色网状结构,在近细胞端纤维排列较致密,含大量血小板。吉姆萨染色见淡蓝色的胶原纤维为网状结构,近细胞端见染为蓝色的白细胞。SEM发现胶原纤维排列成疏松多孔网状支架结构,含大量静止或伸出伪足的血小板及白细胞。TEM发现血小板处于静止或激活状态,α-颗粒释放于细胞和纤维之间,在细胞之间见高密度区为血小板释放的颗粒。结论 PRF胶原纤维的网状立体结构和富含血小板激活释放生长因子,对生物学作用发挥有重要意义。
Objective To investigate the morphological ultrastructure of platelet rich fibrin（PRF）andits implication. Methods PRF was prepared from venous blood samples from 10 healthy donors. ThePRF was observed by light microscopy （H.E staining and Wright Giemsa staining）, scanning electronmicroscopy （SEM） and transmission electron microscopy （TEM）, respectively. Results PRF presentedas light yellow fibrin gel. HE staining showed that collagen fiber was of uniform and light red networkstructure. In the near cell end, the fibers were denser and contained lots of platelets. Giemsa stainingshowed that the collagen fibers were of light blue mesh structure, and the dark blue white blood cellswere in the near cell end. SEM observation found that collagen fibers were arranged in a porous reticularscaffold, containing a large number of static or protruding platelets and leukocytes. TEM showed thatplatelets were at rest or activated, α.particles were released between platelets or fibers, which displayed ahigh density area between cells. Conclusion Through the reticular stereoscopic structure of collagenfibers and rich platelets activating growth factor release, PRF plays an important biological role.
作者
陈铁楼
江一峰
张新海
陈骏
黄传梅
王晓熙
CHEN Tie-lou;JIANG Yi-feng;ZHANG Xin-hai;CHEN Jun;HUANG Chuan-mei;WANG Xiao-xi(Dept.of Periodontal,Oral Research Center of CPLA,Changhai Hospital of PLA Naval Medical University,Shanghai 200081,China;Precision Medical Center,Changhai Hospital of PLA Naval Medical University,Shanghai 200081,China;Dept.of Pathology,Changhai Hospital of PLA Naval Medical University,Shanghai 200081,China)
出处
《同济大学学报（医学版）》
CAS
2018年第4期46-50,共5页
Journal of Tongji University(Medical Science)
基金
上海市卫计委项目(20134418)
中国人民解放军总后勤部项目(CHJ13J035)
关键词
富血小板纤维蛋白
形态学
超微结构
扫描电镜
透射电镜
platelet rich fibrin
morphology
ultrastructure
scanning electron microscope
transmission electron microscope
