摘要
全波长扫描式多功能读数仪(酶标仪)是一种多通道光学系统的分光光度计,将靛酚蓝比色法与酶标仪相结合,建立了酶标仪-靛酚蓝测定水中铵态氮的方法,方法检出限为0.046 mg/L。方法的加标回收率在90.7%~101.8%之间,该方法与连续流动分析仪-水杨酸分光光度法相比,两者测定数据之间回归直线方程为Y(连续流动分析仪-NH_4^+-N)=1.052 4X(酶标仪-NH_4^+-N)-0.009,相关系数R=0.976 1**(n=32,P<0.01)。5个水样重复6次测定,测定结果的相对标准偏差均小于3%。全波长扫描式多功能读数仪(酶标仪)结合靛酚蓝比色法测定重现性好,结果准确,快速方便,可用于水样中铵态氮含量测定。
The indophenol-blue colorimetric method combined with a microplate reader with standard 96-well microplate format was developed to determine ammonium in water samples. The detection limit was 0. 046 mg/L. Values of recovery were in the range of 90. 7% ~ 101. 8% when adding the standard NH_4~＋-N solution to five water samples. The NH_4~＋-N values measured by the colorimetric method with microplate reader（ X） were correlated with by continuous flow analysis and salicylic acid spectrophotometry（ Y）. The regression equation was Y = 1. 052 4 X-0. 009. The correlation coefficient was 0. 976 1＊＊（n = 32,P 0. 01） and linear slope was near 1. 0. The relative standard deviation（ RSD） of proposed colorimetric method with microplate reader was less than 3%（ n = 6）. It is suggested that the indophenol blue microcolorimetric assays based on a standard96-well microplate format and microplate reader could be used to simplify the quantification of ammonium in water samples.
作者
郭文淼
辛宇
张金尧
汪洪
GUO Wen-miao;XIN Yu;ZHANG Jin-yao;WANG Hong(Faculty of Chemistry,Chemical Engineering and En-vironment,Liaoning University of Petro-chemical Engineering,Fushun Liaoning 113001;Institute of Agriculture Resources and Regional Planning,Chinese Academy of Agriculture Sciences,Beijing 100081)
出处
《中国土壤与肥料》
CAS
CSCD
北大核心
2018年第4期166-170,共5页
Soil and Fertilizer Sciences in China
基金
国家重点研发计划项目(2016YFD0200108)
中央级公益性科研院所基本科研业务费专项(Y2017PT14)
