摘要
【目的】研究大豆苷元对人成骨细胞经典的雌激素受体(estrogen receptor,ER)信号通路的调节作用。【方法】以同时表达两种ER亚型的人MG-63成骨样细胞为研究模型,分别应用不同终浓度的大豆苷元(0.01、0.1、1μmol/L)、雌二醇(0.1μmol/L)、雷洛昔芬(0.1μmol/L)或二甲基亚砜(dimethyl sulfoxide,DMSO)处理,通过荧光素酶报告基因、RT-PCR及免疫印迹等技术,观察大豆苷元对MG-63细胞经典的雌激素反应元件(estrogen response element,ERE)的基因转录活性、ER亚型及其相关的类固醇受体共激活因子(steroid receptor coactivator,SRC-1)表达的调节作用。【结果】大豆苷元可增强MG-63细胞经典的ERE基因转录活性,同时使其ERα、ERβ及SRC-1表达水平升高。【结论】大豆苷元可通过ER经典的基因组作用机制,即增强ERE基因转录活性对成骨细胞发挥效应,其机制可能与其增加ERα、ERβ及其相关的共激活因子SRC-1的表达水平相关。
【Objective】To study the regulation of daidzein on the classical estrogen receptor（ER） signal pathway in human osteoblastlike MG-63 cells.【Methods】Daidzein（0.01、0.1、1 μmol/L）, estradiol（0.1 μmol/L）, raloxifene（0.1 μmol/L） or dimethyl sulfoxide with different concentration was added to the human osteoblast-like MG-63 cells expressing two types of ER subtypes.. The regulation of daidzein on the classical estrogen response element（ERE） transcriptional activity, the expression of ER subtypes and related steroid receptor coactivator（SRC）-1 in MG-63 cells were investigated by luciferase reporter assay, RT-PCR and Western blot.【Results】Daidzein increased activity of the classical ERE gene transcription pathway as well as the expression of ERα, ERβ and SRC-1 in human osteoblastic MG-63 cells.【Conclusion】Daidzein can exert effect on osteoblasts by enhancing the transcriptional activity of ERE gene through the classical genomic action mechanism of ER. The mechanism may be related to the increase of the expression level of ERα, ERβ and relevant co-activating factor SRC-1.
作者
王志平
禹博
刘明皓
苗德宇
赵子义
王越
WANG Zhi-ping;YU Bo;LIU Ming-hao;MIAO De-yu;ZHAO Zi-yi;WANG Yue(Department of Pharmacy,People＇s Hospital of Zhengzhou,Zhengzhou 450001,China)
出处
《武警后勤学院学报（医学版）》
CAS
2018年第3期200-203,共4页
Journal of Logistics University of PAP（Medical Sciences）
基金
国家自然科学基金项目(81273520
81572852)
天津市自然科学基金项目(12JCZDJC26300)
