摘要
以‘富士’苹果为对照,研究了4种海棠果实提取物的总多酚含量和对人胃癌细胞BGC-803的体外抑制活性。用福林-酚法测定总多酚含量,采用高效液相色谱法测定单体酚种类和含量,用细胞活力检测试剂盒和倒置显微镜检测对人胃癌细胞BGC-803的抑制作用。结果表明:5种果实提取物总多酚含量变化范围为405.99~4663.30 mg GAE/100 g,海棠果实提取物总多酚含量均高于‘富士’苹果;5种果实多酚提取物对人胃癌细胞BGC-803的抑制作用(IC50值)比较结果显示,红果肉的‘亚当’海棠(42.29μg/m L)和‘红丽’海棠(64.67μg/m L)优于黄果肉的‘海红果’海棠(1466μg/m L)和西府海棠(2291μg/m L),4种海棠均优于‘富士’苹果(3476μg/m L);5种果实多酚提取物中单体酚主要有原花青素B1、原花青素B2、儿茶素、表儿茶素等;两种红果肉海棠(‘亚当’海棠、‘红丽’海棠)中,以矢车菊素半乳糖苷和低聚原花青素类为主的多酚类物质含量高于黄果肉的‘海红果’海棠、西府海棠和‘富士’苹果。
Took Malus domestica ‘Fuji＇as control,the total phenolic contents（ TPC） and in vitro inhibition effect（ VIE） on human gastric BGC-803 cell lines of four crabapples fruits extracts were detected.TPC were determined using Folin-Ciocalteu reagent.Individual phenolic compounds were determined by high performance liquid chromatography（ HPLC）. Inhibition effect on human gastric BGC-803 cell lines was detected using Cell Counting Kit-8（ CCK-8） and inverted microscope. The results showed that the TPC range of five fruits extracts was 405.99 - 4663.30 mg GAE/100 g,in which the TPC of crabapples were higher than Malus domestica ‘Fuji＇.The results of inhibitory effect（ IC50 value） of five fruits extracts on human gastric BGC-803 cell lines showed that red-fleshed Malus‘Adams＇（ 42.29 μg/mL） and Malus‘Red splendor＇（ 64.67 μg/mL） were better than yellow-fleshed Malus micromalus ‘Haihongguo ＇（ 1466 μg/mL） and Malus micromalus（ 2291 μg/mL）,and four crabapples were all better than Malus domestica ‘Fuji ＇（ 3476 μg/mL）. The individual phenolics of five extracts mainly contained procyanidins B1,procyanidins B2,（ ＋）-catechin,and（-）-epicatechin. Malus ‘Adams＇ and Malus ‘Red splendor＇,which were varieties with red flesh,had a higher content of cyanidin-3-galactoside and oligo procyanidins than yellow-fleshed Malus micromalus ‘Haihongguo＇,Malus micromalus,and Malus domestica ‘Fuji＇.
作者
李嫒
李厚华
刘小微
韩美玲
毛沛琪
LI Ai;LI Hou-hua;LIU Xiao-wei;HAN Mei-ling;MAO Pei-qi(College of Landscape Architecture and Arts,Northwest A＆F University,Yangling 712100,China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第18期279-284,共6页
Science and Technology of Food Industry
基金
中央财政拨款林业推广项目(陕林SLTG201603)
国家自然科学基金(31570697)
关键词
海棠果
多酚
抗癌
相关性
crabapple
phonohc
anticancer effect
correlation
