摘要
目的探讨RNA干扰抑制CD151基因表达对宣威肺腺癌XWLC-05细胞侵袭和迁移的影响及裸鼠肺转移的实验。方法构建能高效抑制CD151基因表达的3组重组干扰质粒,筛选出稳定转染干扰质粒的宣威肺腺癌XWLC-05细胞株。MTT、划痕实验、Transwell实验研究干扰CD151基因表达前后对细胞迁移和侵袭的影响。将构建的RNA干扰抑制CD151基因表达的稳转细胞XWLC-05-shRNA、XWLC-05-shNC、XWLC-05-Blank通过尾静脉注射入BALB/c裸鼠,30天后通过肉眼和HE染色后观察肺转移情况。结果划痕实验和Transwell实验表明RNA干扰抑制CD151基因表达可能抑制宣威肺腺癌XWLC-05细胞体外迁移和侵袭作用(P<0.05)。XWLC-05-shRNA、XWLC-05-shNC、XWLC-05-Blank各组裸鼠肺转移率及肺组织上的转移病灶数依次为75%(6/8)与(5.2±1.2)、100%(8/8)与(9.8±1.6)、87.5%(7/8)与(9.1±1.2)。XWLC-05-shNC与XWLC-05-Blank组比较差异无统计学意义(P=0.423),XWLC-05-shRNA与XWLC-05-shNC(P=0.000)和XWLC-05-Blank组比较差异有统计学意义(P=0.000)。结论 RNA干扰抑制CD151基因表达能抑制XWLC-05细胞的体外迁移和侵袭;能减少XWLC-05细胞裸鼠的肺转移。CD151可能成为抑制宣威肺癌侵袭和迁移的潜在治疗靶点之一。
Objective To explore the influence of CD151 inhibition by RNA interference on invasion and metastasis of Xuanwei lung adenocarcinoma XWLC-05 cells,as well as lung metastasis in nude mice given tail veins injection of XWLC-05 cells. Methods Recombinant plasmid aimed to interfere CD151 gene was constructed and transfected into XWLC-05 cells. MTT,wound-healing assay and Transwell assay were applied to detect cell proliferation and migration abilities. Stably-expressed XWLC-05-shRNA,XWLC-05-shNC and XWLC-05-Blank cell lines were injected into the tail veins of BALB/c nude mice. Lung metastasis was measured by naked eye and microscope after HE staining. Results Inhibition of CD151 gene expression could reduce the migration and invasion abilities of XWLC-05 cells in vitro（P0.05）. The proportion and number of lung metastasis were 75%（6/8） and（5.2±1.2）,100%（8/8） and（9.8±1.6）,75%（7/8） and（9.1±1.2） in XWLC-05-shRNA,XWLC-05-shNC and XWLC-05-Blank groups,respectively. There was no differences in XWLC-05-shNC vs. XWLC-05-Blank group（P=0.423）. There were significant differences in XWLC-05-shRNA vs. XWLC-05-shNC group（P=0.000） and XWLC-05-shRNA vs. XWLC-05-Blank group（P=0.000）. Conclusion Inhibition of CD151 gene expression could reduce lung metastasis in nude mice given tail veins injection of XWLC-05 cells. CD151 may become one of the potential therapeutic targets for inhibiting the invasion and metastasis of Xuanwei lung adenocarcinoma.
作者
李科
汪矗
杨芳
曾佳佳
张建强
LI Ke;WANG Chu;YANG Fang;ZENG Jiajia;ZHANG Jianqiang(Medical Oncology,The Third Affiliated Hospital of Kunming Medical University,Yunnan Province Tumor Hospital,Kunming 650118,China;Department of Respiratory Medicine,The First Affiliated Hospital of Kunming Medical University,Kunming 650031,China;Department of Biology and Chemistry,Puer College,Puer 665000,China)
出处
《肿瘤防治研究》
CAS
CSCD
2018年第9期623-628,共6页
Cancer Research on Prevention and Treatment
基金
2015年云南省教育厅科学研究基金(2015Y181)
2015年云南省肿瘤医院博士科研基金(BSJJ201506)
2017年普洱学院校级创新团队<超分子生物活性材料团队>(CXTD14)
普洱学院高层次人才科研启动经费(K2017051)
