摘要
目的探讨原发性高血压患者血清miRNA-663水平与肾素-血管紧张素-醛固酮系统(RAAS)生化标志物的相关性。方法受试者分为原发性高血压组、健康对照组,每组50例。分别采用荧光实时定量聚合酶链反应、化学比色法及放射免疫法检测外周血血清miRNA-663、血管紧张素转换酶(ACE)活性,以及肾素(RA)、血管紧张素(Ang)Ⅱ、醛固酮(ALD)的表达。结果相对于健康对照组,原发性高血压组患者外周血血清miRNA-663表达显著降低,ACE活性及RA、AngⅡ、ALD表达均显著升高(P<0.01);原发性高血压患者血清miRNA-663水平与血清ACE活性及RA、AngⅡ、ALD水平均呈显著负相关。结论原发性高血压患者外周血血清miRNA-663表达显著下调,RASS生化标志物显著升高,外周血血清miRNA-663表达与RASS生化标志物呈显著负相关关系。
Objective To investigate the correlation between serum miRNA-663 level and biochemical markers of renin-angiotensin-aldosterone system （RAAS） in the patients with essential hypertension. Methods The subjects were divided into the primary hypertension group and healthy control group,50 cases in each group.The levels of peripheral serum miRNA-663,angiotensin converting enzyme （ACE） activity and the expressions of rennin （RA）,AngⅡ and aldosterone （ALD） were detected by adopting qRT-PCR,chemical colorimetric and radioimmunoassay methods,respectively. Results Compared with the healthy control group,the expression of peripheral serum miRNA-663 in the patients with PH was decreased significantly （ P 〈 0.01 ）,and the ACE activity and expressions of RA,AngⅡ and ALD were increased significantly （ P 〈0.01）.The level of serum miRNA-663 had significantly negative correlation with serum ACE activity and levels of RA,AngⅡand ALD in the patients with primary hypertension. Conclusion The expression of peripheral serum miRNA-663 is significantly decreased,and biochemical markers of RAAS are significantly increased in the patients with primary hypertension.The peripheral serum miRNA-663 expression has significantly negative correlation with the biochemical markers of RAAS.
作者
刘文果
陈石磊
万志佳
魏星
LIU Wenguo;CHEN Shilei;WAN Zhijia;WEI Xing(Department of Clinical Laboratory,Chongqing Municipal Institute of Tuberculosis Treatment and Prevention,Chongqing 400050,China;Department of Preventive Medicine,Military Preventive Medical College,Military Medical University,Chongqing 400038,China;Department of Clinical Laboratory,Shapingba District Maternal and Child Health Care Family Planning Service Center,Chongqing 400030,China;Department of Clinical Laboratory,Fengjie County People′s Hospital,Chongqing 404600,China)
出处
《检验医学与临床》
CAS
2018年第19期2899-2901,2905,共4页
Laboratory Medicine and Clinic
