摘要
目的探讨垂体肿瘤转化基因1(PTTG1)和神经胶质瘤相关癌基因1(Gli1)在脑膜瘤中的表达水平及其与患者临床病理特征和预后的关系。方法选择2010年10月至2014年7月神经外科确诊的脑膜瘤患者98例,同期选择72例脑膜瘤癌旁组织作为对照组,采用酶联免疫吸附法测定脑膜瘤组织及癌旁组织PTTG1、Gli1蛋白含量、免疫组化法测定PTTG1、Gli1蛋白在脑膜瘤组织及癌旁组织中的表达,分析PTTG1、Gli1蛋白的表达情况与脑膜瘤患者临床病理特征及脑膜瘤患者预后的关系。结果脑膜瘤组织PTTG1、Gli1蛋白水平高于癌旁组织,差异均有统计学意义(t=13. 474、14. 859,P <0. 01);脑膜瘤组织PTTG1、Gli1阳性率高于癌旁组织,差异有统计学意义(P <0. 01);脑膜瘤组织PTTG1、Gli1蛋白表达水平不同年龄间比较差异无统计学意义(P> 0. 05);不同病理学分级、TNM分期、浸润深度及有无淋巴血管间隙浸润和淋巴结转移者的PTTG1、Gli1蛋白表达差异有统计学意义(P <0. 05); PTTG1、Gli1阴性组3年生存率及生存期均明显高于PTTG1、Gli1阳性组,差异有统计学意义(χ2=14. 654、13. 875,t=13. 097、12. 982,P <0. 05)。结论 PTTG1、Gli1蛋白在脑膜瘤组织的表达量高于癌旁组织,PTTG1、Gli1蛋白在脑膜瘤的发生发展过程中起促进作用; PTTG1、Gli1蛋白阴性表达者能获得较好的预后。
Objective To investigate the expression levels of pituitary tumor transforming gene 1（PTTG1） and glioma related oncogene 1（Gli1） in meningioma and the clinical significance. Methods The meningioma tissues of 98 patients with meningioma who were diagnosed as meningioma in neurosurgery department of Second Affiliated Hospital of Hainan Medical University from October 2010 to July 2014 and 72 cases of para-cancerous tissues（control group） at the same period were selected. Enzyme linked immunosorbent assay（ELISA） was used to detect contents of PTTG1 and Gli1 proteins in meningioma tissues and para-cancerous tissues. Immunohistochemical method was used to detect the expressions of PTTG1 and Gli1 proteins. The associations of the expressions of PTTG1 and Gli1 proteins and the clinicopathological features and prognosis of meningioma patients were analyzed. Results The PTTG1 and Gli1 proteins contents in meningioma tissues were significantly higher than those in para-cancerous tissues（t = 13. 474,14. 859,P〈0. 05）. The positive expression rates of PTTG1 and Gli1 proteins in meningioma tissues were significantly higher than those in paracancerous tissue（χ2= 25. 847,27. 399,P〈0. 05）. There were no significant differences in expressions of PTTG1 and Gli1 proteins among different age groups（all P〈0. 05）. There were significant differences in the expressions of PTTG1 and Gli1 proteins for different pathological grading,different TMN staging,different depth of invasion,with or without lymphvascular space invasion and with or without lymph node metastasis（all P〈0. 05）. The 3-year survival rate and survival period in PTTG1 and Gli1-negative groups were significantly higher than those in PTTG1 and Gli1-positive groups（χ2=14. 654,13. 875,t = 13. 097,12. 982; all P〈0. 05）. Conclusion The expression levels of PTTG1 and Gli1 proteins in meningioma tissues are higher than those in para-cancerous tissues which suggests that PTTG1 and Gli1 proteins play a promoting role in the occurrence and development of meningioma,and PTTG1 and Gli1 proteins negative expressions can achieve better prognosis.
作者
何青龙
欧阳一彬
刘达远
莫业和
HE Qing-long, OUYANG Yi-bin, LIU Da-yuan, MO Ye-he(Department of Neurosurgery, Second Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, China)
出处
《中国临床研究》
CAS
2018年第9期1161-1164,1169,共5页
Chinese Journal of Clinical Research
基金
海南省自然科学基金(817254)
