摘要
目的：探究忍冬枝枯病对金银花产量、金银花和忍冬藤有效成分含量的影响,为忍冬枝枯病的防治和药材质量评价提供科学依据。方法：在金银花头茬花期比较各病级忍冬植株平均单株结蕾量,并称量花蕾的鲜重、干重;按照2015年版《中国药典》测定各病级忍冬植株金银花样品中绿原酸和木犀草苷的含量以及忍冬藤样品中绿原酸和马钱苷的含量。结果：忍冬0~7级植株平均单株结蕾量、鲜重、干重差异极显著（P〈0.01）,其中健株结蕾量最多,7级植株结蕾量最少。病级越高,植株结蕾量越少,产量越低。各病级忍冬植株金银花样品绿原酸和木犀草苷以及忍冬藤样品绿原酸和马钱苷的含量均达到2015年版《中国药典》标准。金银花中绿原酸的质量分数为2.31%~3.46%,其中1级病树金银花中绿原酸的含量最高,健株（0级）金银花中绿原酸的含量最低。各发病等级植株金银花中绿原酸含量均高于健株,差异极显著（P〈0.01）。金银花中木犀草苷的质量分数为0.050%~0.065%,健株金银花中木犀草苷的含量最低。健株和其他各发病等级植株金银花中木犀草苷含量差异极显著（P〈0.01）。忍冬藤中绿原酸的质量分数为0.43%~0.54%,健康植株忍冬藤中绿原酸的含量高于其他各发病等级植株,差异极显著（P〈0.01）。忍冬藤中马钱苷的质量分数为0.82%~1.58%,各发病等级植株忍冬藤中马钱苷含量均低于健康植株,差异极显著（P〈0.01）。结论：忍冬枝枯病的发生,有利于金银花中绿原酸和木犀草苷的积累,但会显著降低忍冬藤中绿原酸和马钱苷的含量以及金银花的产量。
Objective： To investigate the effect of branch blight on the yield of honeysuckle as well as the yield and the content of active components of Lonicera japonica,and provide scientific basis for field management on branch blight disease and quality evaluation. Method： The yield of honeysuckle was measured by the number of buds and their total weight harvested at the first full-bloom stage. The contents of chlorogenic acid and luteolin-7-O-glucosid in honeysuckle samples andthe contents of chlorogenic acid and loganin in the L. japonica caulis from all grades of L. japonica were measured according to method illustrated in Chinese Pharmacopoeia（ 2015）. Result：The average number,fresh weight and dry weight of buds per plant from grade 0 grade 7 were significantly different（ P〈0. 01）. Among them,healthy plants had the highest quantity of buds and grade 7 plant had the lowest quantity. The higher the disease grade,the smaller the amount of buds and the lower the yield. The contents of active components including chlorogenic acid and luteolin-7-O-glucosid in honeysuckle samples andchlorogenic acid and loganin in the L. japonica caulis from all disease grades were in line with the standards in Chinese Pharmacopoeia（ 2015）. The content of chlorogenic acid in all honeysuckle samples ranged from 2. 31% to3. 46%,among which the content of chlorogenic acid in the honeysuckle sample from grade 1 of diseased tree was the highest,while that from grade 0（ healthy plant） was the lowest. The content of chlorogenic acid in the samples from diseased tree was significantly higher than that from hea1 thy plants（ P〈0. 01）. The content of luteolin-7-Oglucosid in honeysuckle samples ranged from 0. 050% to 0. 065%, among which the content of luteolin-7-Oglucosid from the grade 0 honeysuckle sample was the lowest. The content of luteolin-7-O-glucosid in healthy plants was significantly different from other disease grades（ P〈0. 01）. The content of chlorogenic acid in L. japonica caulis was 0. 43% to 0. 54%,and the content of chlorogenic acid in healthy plants was higher than that in other disease-grade plants（ P〈0. 01）. The content of loganin in L. japonica caulis was 0. 82% to 1. 58%,and the content of loganin in all diseased sample plants of L. japonica caulis was significantly lower than that in healthy ones（ P〈0. 01）. Conclusion： The branch blight disease was beneficial to the accumulation of chlorogenic acid and Luteolin in honeysuckle,but could significantly decrease the yield and the contents of chlorogenic acid and loganin in the L. japonica caulis.
作者
杨帆
刘玉霞
李炯
高素霞
文艺
王飞
鲁传涛
刘红彦
YANG Fan;LIU Yu-xia;LI Jiong;GAO Su-xia;WEN Yi;WANG Fei;LU Chuan-tao;LIU Hong-yan(Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Chinese Herbal Medicine Production Technology Service Center,Department of Agricultural of Henan Province,Zhengzhou 450002,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第20期32-37,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中药材产业技术体系(CARS-21)
河南省农业科学院自主创新专项(2017ZC44)
关键词
忍冬
枝枯病
金银花
忍冬藤
产量
Lonicera japonica
branch blight
honeysuckle
Lonicera japonica caulis
yield
