摘要
目的观察石甘散及其主要药物甘松、石菖蒲对戊四氮致痫大鼠氧化应激反应的影响,并根据核因子E2相关因子2(Nrf2)和血红素加氧酶1(HO-1)因子的变化研究石甘散可能的抗氧化作用机制。方法除空白组外,将点燃成功的戊四氮致痫大鼠随机分为模型组、西药组(丙戊酸钠灌胃治疗)、甘松组、石菖蒲组和石甘散组。所有大鼠经14 d干预后处死,取大鼠脑部海马组织,考马斯亮蓝法测定海马组织超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)蛋白含量,Western bloting法测定海马组织Nrf2和HO-1蛋白含量,RT-PCR法测定海马组织Nrf2 m RNA表达、HO-1 m RNA表达。结果 1)癫痫模型点燃成功后,大鼠海马组织SOD、GSH-Px含量明显降低,MDA含量明显升高(P<0.01)。丙戊酸钠、甘松、石菖蒲、石甘散均可以增加致痫大鼠SOD、GSH-Px含量、降低MDA含量,丙戊酸钠、石甘散作用效果强于甘松、石菖蒲单独应用(P<0.05或P<0.01),且两组间差异无统计学意义(P>0.05);2)癫痫模型点燃后,大鼠海马组织Nrf2、HO-1蛋白含量均升高(P<0.05或P<0.01)。丙戊酸钠、甘松、石菖蒲、石甘散均能进一步增加Nrf2、HO-1蛋白含量,西药组、石甘散组Nrf2、HO-1蛋白的增加明显高于甘松、石菖蒲单独应用(P<0.05或P<0.01),且两组间效果差异无统计学意义(P>0.05);3)癫痫模型点燃后,大鼠海马组织Nrf2 m RNA表达、HO-1 m RNA含量升高,丙戊酸钠、甘松、石菖蒲、石甘散均可以进一步使Nrf2 m RNA表达、HO-1 m RNA表达增加(P<0.05或P<0.01),且各治疗组间提高m RNA表达效果差异无统计学意义(P>0.05)。结论石甘散及其配方组成甘松、石菖蒲均具有抗戊四氮致痫大鼠氧化应激损伤、清除自由基的作用,其作用效果可能与激活Nrf2/HO-1信号通路中Nrf2因子并促进下游HO-1因子表达相关,甘松、石菖蒲的合成方石甘散作用效果最显著。
Objective： To observe the effects of Shigan Decoction and its main drug-rhizoma nardostachyos and acorus gramineus soland on oxidative stress reaction of mice with epilepsy induced by PTZ,and to study its possi- ble antioxidant meehanism,according to the changes of Nrf2 and HO-1. Methods： Mice with epilepsy induced by sueeessfhlly lighted PTZ were randomly divided into the model group,Western medicine group （treated by sodium valproate）,Gansong group,Shiehangpu group and Shigan Decoction group. All rats were killed after 14 days＂ in- tervention,and the hippocampal tissues of rats were taken. Coomassie brilliant blue method was used to detect the protein content of SOD,MDA and GSH-Px in hippocampal tissues;Western bloting method was used to detect the protein content of Nrt2 and HO-1 ;RT-PCR method was used to detect the mRNA expression of Nrt2 and HO-1. Results： 1）After the epilepsy models were lighted sueeessfhlly,the contents of SOD and GSH-Px declined,and MDA increased obviously （P〈 0.01 ）. Sodium valproate, Gansong,Shiehangpu and Shigan Decoction all could raise the content of SOD and GSH-Px,reducing MDA,Sodium valproate and Shigcm Decoction had a better effeet than Gansong and Shiehangpu alone,with no significant difterenee （P〉 0.05）. 2）After the epilepsy models were sue- eessfhlly lighted,the protein content of Nrt2 and HO-1 both increased. Sodium valproate,Gansong,Shiehangpu and Shigan Decoction all could further raise the protein content of Nrf2 and HO-1 （P〈0.05 or P〈0.01）. West- em medicine group and Shigan Decoction group had a higher increase than Gansong and Shichangpu alone,with no significant difference （P〉 0.05）. 3）After the epilepsy models were successfully induced,mRNA expression of Nrf2 and HO-1 both increased（P〈 0.05 or P〈 0.01 ）. Sodium valproate, Gansong, Shichangpu and Shigan Decoc- tion all could further increaase the mRNA expression,with no difference among all treatment groups. Conclusion： Shigan Decoction and its components Gansong and Shichangu can strengthen the antioxidant effects in PTZ in- duced rats,which may be related to activating factor Nrf2 in Nrf2/HO-1 signal pathways and promoting the ex- pression of HO-1. Shigan Decoction with components of Gansong and Shichangpu has the most significant effects than single drug.
作者
庞博
张韧
张奇
王轩
程为平
Pang Bo;Zhang Ren;Zhcalg Qi(Heilongjiang University of Chi-nese Medicine,Heilongjiang,Harbin 150040,China.)
出处
《中国中医急症》
2018年第10期1722-1725,1729,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
黑龙江省自然科学基金项目(H201470)
