摘要
目的：研究＂钻类＂瑶族药＂十八钻＂之一的黄红钻乙酸乙酯萃取部位的化学成分。方法：黄红钻干燥药材经粉碎,用70%乙醇水进行回流提取,得到粗提物,粗提物加适量的水分散,分别用石油醚、乙酸乙酯、正丁醇进行萃取,对其中乙酸乙酯萃取部位采用反复硅胶柱色谱法、高效制备液相进行分离纯化,通过化合物的谱学数据分析（包括1H-NMR,13C-NMR,MS）,以及比对文献数据进行结构鉴定及确证。结果：从黄红钻乙酸乙酯萃取部位分离出11个化合物,分别鉴定为对羟基苯甲醛（1）,香草醛（2）,松柏醛（3）,4-羟基苯乙基乙酸酯（4）,反式肉桂酸（5）,反式阿魏酸（6）,丁香酸乙酯（7）,4-羟基-3,5二甲氧基苯甲醛（8）,反式阿魏酸乙酯（9）,4-羟基-3-甲氧基苯甲酸乙酯（10）,邻苯二甲酸-二（2-乙基-己基）酯（11）。结论：从黄红钻中分离得到的化合物大部分为小分子的酚酸类化合物,且所有的化合物均为从黄红钻中首次分离得到。以上结果为黄红钻药效物质基础的研究提供了依据。
Objective： To study the chemical constituents of ethyl acetate extract from one of the＂Shi ba zuan＂of Yao medicines,Jasminum pentaneurum. Method： The crude extract of dry powder from stems of J.pentaneurum was obtained by using 70% ethanol water refluxing; the extract was evaporated under vacuum to obtain the dry residue. Then, the residue was dissolved in water and extracted with petroleum ether, ethyl acetate,and n-butanol,respectively. The compounds from the ethyl acetate fraction were isolated and purified by repeated silica gel column and high performance liquid chromatography（ HPLC）, and their structures were determined by spectroscopic data analyses,including1 H-NMR,13 C-NMR,and MS,as well as by comparison with the data from literature. Result： Eleven compounds were obtained and identified as 4-hydroxybenzyl aldehyde（ 1）,vanillin（ 2）,coniferaldehyde（ 3）,4-hydroxy phenethyl acetate（ 4）,trans-cinnamic acid（ 5）,transferulic acid（ 6）, syringic acid ethyl ester（ 7）, 4-hydroxy-3, 5-dimethoxybenzaldehyde（ 8）, trans-ethyl ferulate（ 9）,ethyl 4-hydroxy-3-methoxybenzoate（ 10）,and bis（ 2-ethylhexyl） phthalate（ 11）,respectively.Conclusion： All the compounds were isolated from J. pentaneurum for the first time,and most of the isolated compounds were phenolic acids with small molecules. The results provide a basis for the efficacy basis study of J. pentaneurum.
作者
韦玮
邓家刚
郝二伟
秦健峰
谢金玲
罗花
邓艳台
陈诗茵
张思鳗
侯小涛
WEI Wei;DENG Jia-gang;HAO Er-wei;QIN Jian-feng;XIE Jin-ling;LUO Hua;DENG Yan-tai;CHEN Shi-yin;ZHANG Si-man(Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Guangxi Collaborative Innovation Center on Functional Ingredients of Agricultural Residues,Guangxi University of Chinese Medicine,Nanning 530200,China;School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第21期51-55,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西中医药大学2017年引进博士科研启动基金项目(2017BS044)
2017年广西科技计划项目(2017AD19024)
广西重点实验室建设项目(17-259-20)
广西农作物废弃物功能成分研究协同创新中心建设项目(CICAR 2017-Z1)
广西科技基地与人才专项(桂科AB17195019)
关键词
黄红钻
木犀科
化学成分
分离鉴定
Jasminum pentaneurum
Oleaceae
chemical constituents
isolation and identification
