摘要
氨基酸是构成茶叶滋味的重要组成部分,茶汤的品质与其含量和成分的比例有关,为了更好地研究茶汤的品质,实验建立了一种快速检测茶叶中游离氨基酸的氯甲酸-9芴基甲酯(FMOCCl)柱前衍生-反相高效液相色谱(RP-HPLC)分析方法。该方法的色谱条件为:Phenomenex Kinetex C18反相色谱柱(100 mm×2.1 mm,2.6μm);流动相包含2部分,A相为含0.05 mol/L乙酸钠的水溶液(pH5.28),B相100%乙腈,梯度洗脱;流速0.15 mL/min;柱温(35±1)℃;紫外检测波长262nm,进样体积10μL时,在(0.004～1)mg/mL浓度范围内,19种氨基酸质量浓度与峰面积呈良好的线性关系,线性相关系数0.988～0.999,检出限(LOD,S/N≥3)范围(0.4～300)μg/mL,定量限(LOQ,S/N≥10)范围(0.8～500)μg/mL,且平均回收率90.29%～105.38%,相对标准偏差(RSD)范围0.36%～15.38%。应用此方法比较分析了不同香型凤凰单丛茶中19种游离氨基酸含量。
Amino acid is an important component of tea taste. The aim of this work was to establish a method for the quality of tea by pre-column derivatization reversed high performance liquid chromatography（RP-HPLC）. The samples were derived by 9-fluorenylmethylchloroformate（FMOC-Cl）and HPLC method. Samples were injected with 10 μL into a reverse phase Phenomenex Kinetex C18 column（100 mm×2.1 mm, 2.6 μm）. The column operated at（35±1）℃ with a flow rate of 0.15 mL/min using 50 mmol/L acetate buffer（pH5.28） as eluent A and 100% acetonitrile as eluent B. The detection wavelength was operated at 262 nm. The calibration curves had good linearity over concentration range of（0.004-1）mg/mL. The linear relative coefficient for 19 amino acids over concentration range of（0.004-1）mg/mL were 0.988-0.999. The limit of detectins（LOD, S/N≥3） were（0.4-300）μg/mL,and limit of quantifications（LOQ, S/N≥10） were（0.8-500）μg/mL. The mean recovery ratio were90.29%-105.38% with RSDs of 0.36%-15.38%. The 19 free amino acids in different flavor Fenghuang single tea（a kind of Oolong tea） were analyzed by this method.
作者
孔谦
陈栋
陈庄
吴华玲
史敬芳
黄文洁
晏石娟
KONG Qian;CHEN Dong;CHEN Zhuang;WU Hua-ling;SHI Jing-fang;HUANG Wen-jie;YAN Shi-juan(.Agrobiological Gene Research Center, Guangdong Academy of Agricultural Sciences Guangzhou 510640; .Guangdong Key Laboratory of Tea Plant Resources Innovation and Utilization, Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 51064)
出处
《食品科技》
CAS
北大核心
2018年第10期320-325,共6页
Food Science and Technology
基金
广东省农业领域科技计划项目(2013B020305005,2015A020209076)
国家现代农业茶叶产业技术体系专项资金项目(CARS-23)
关键词
茶叶
FMOC-Cl柱前衍生
反相高效液相色谱法
游离氨基酸
tea
pre-column derivatization with 9-fluorenylmethylchloroformate （FMOC-Cl）
reversed highperformance liquid chromatography
free amino acid
