摘要
目的:了解肉类食品中金黄色葡萄球菌的耐药性等情况。方法:利用GB 4789.10-2016增菌分离法获取金黄色葡萄球菌,通过K-B法检测样本的药敏性,肠毒素PCR基因检测对肠毒素情况进行检测,利用琼脂法筛选出MRSA菌株,并且对MRSA进行生化试验检测。结果:在获取的110份生肉样品中,其中检测得到35份样品中含有金黄色葡萄球菌,检出率为31.81%;对这35株金黄色葡萄球菌的12种抗生素耐药性进行检测,可知其对青霉素的耐药性达到100%,四环素以及甲氧苄胺嘧啶的耐药率也分别高达88.57%和77.14%,具有非常严重的耐药性。对于6种或者6种以上的多重耐药率达到48.58%,肠毒素检出率为34.28%,MRSA检出率为34.28%。
Objective：To understand the drug resistance of Staphylococcus aureus in meat foods. Methods：Staphylococcus aureus was obtained by enrichment and isolation method of GB 4789.10-2016. The drug sensitivity of the sample was detected by KB method. The enterotoxin was detected by PCR detection of enterotoxin. The MRSA strain was screened by agar method and MRSA was performed. Biochemical test. Results：Among the 110 raw meat samples obtained, 35 samples were found to contain Staphylococcus aureus, and the detection rate was 31.81%. The antibiotic resistance of these 35 strains of Staphylococcus aureus was detected. The resistance to penicillin reached 100%, and the resistance rates of tetracycline and trimethoprim were also as high as 88.57% and 77.14%, respectively, with very serious drug resistance. For 6 or more, the multi-drug resistance rate reached 48.58%, the intestinal toxin detection rate was 34.28%, and the MRSA detection rate was 34.28%.
作者
李昊翔
LI Hao-xiang(Minxi Vocational ＆ Technical College,Longyan 364000,China)
出处
《黑龙江生态工程职业学院学报》
2018年第6期27-28,62,共3页
Journal of Heilongjiang Vocational Institute of Ecological Engineering
关键词
肉类食品
耐药性
金黄色葡萄球菌
检测
MDeat food
Drug resistance
Staphylococcus aureus
Detection
