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培养基对MDCK细胞贴壁率的影响 被引量:2

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摘要 为确定培养基种类对犬肾细胞(MDCK)贴壁率的影响,用DMEM、MEM、M199和RPMI1640四种培养基加10%新生牛血清后分别以10个/cm2的密度接种培养MDCK细胞,培养14 d计数每种培养基培养后形成的细胞集落数,并计算贴壁率。结果表明,四种培养基对MDCK细胞均能形成明显的细胞集落,贴壁率分别为78.56%、60.23%、41.13%和39.61%,以DMEM培养基培养MDCK的贴壁率最高。
出处 《甘肃畜牧兽医》 2018年第10期75-77,共3页 Gansu Animal Husbandry and Veterinary Medicine
基金 国家科技重大专项(2015ZX09102016) 西北民族大学中央高校基本科研业务费专项资金项目(31920150029) "双一流"和特色发展引导专项资金生物工程特色学科(1001070204)
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