摘要
为了解脑损伤后自由基含量的变化及其与脑水肿之间的关系 ,将 4 2只SD大鼠分为正常组、假手术组、手术组 3组。采用黄嘌呤氧化酶法测定损伤灶周围脑组织匀浆中的超氧化物歧化酶 (SOD)活力 ,硫代巴比妥酸法测定丙二醛 (MDA)含量 ,双缩脲法测定组织蛋白 ,干湿法测定脑组织含水量。结果显示 ,假手术组与正常组比较无明显差异 ,颅脑损伤后损伤灶周围脑组织匀浆中SOD活力下降 ,72h达最低值 ,然后缓慢回升 ,但一直低于假手术组与正常组 (P <0 .0 5 ) ;脑组织匀浆中MDA的含量在伤后迅速上升 ,72h达最高值 ,后虽有下降 ,但一直处于较高水平。上述变化与假手术组和正常组比较有明显差异 (P <0 .0 5 )。大鼠脑损伤后损伤灶周围脑组织中SOD在 7d内持续降低 ,MDA持续升高 。
To determine and evaluate the changes of free radicals in brain tissue after brain injury and the relationship between them and brain edema, 42 SD rats were used and divided into three groups: normal controls, sham group and operation group. The cerebral contusion model was produced by weight dropping. The brain injury model was built successfully. The contents of SOD and MDA in cerebral contusion tissue were measured, and so were brain water contents. The data from the normal control group and the sham group showed no significant difference. MDA values of the traumatic right hemisphere of the operation group were significant higher than that of the normal control group and sham group, so were brain water contents (P<0.05). SOD activity of the traumatic hemisphere was lower than that of the other two group (P<0.05). The results of this study suggests: ①lipid peroxidation takes place in the traumatic cerebrum, and free radicals play the role in tissue damage due to ischemia and bleeding; ②oxidative stress causes brain edema and vice versa.
出处
《首都医科大学学报》
CAS
2002年第3期236-239,共4页
Journal of Capital Medical University
